Visualization and Regulation of Tumor Metastasis Based on Cell Surface Azaelectrocyclization and Noninvasive Imaging

2014 Fiscal Year Final Research Report

• Project/Area Number: 23681047
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Living organism molecular science
• Research Institution: The Institute of Physical and Chemical Research (2012-2014); Osaka University (2011)
• Principal Investigator: TANAKA Katsunori 独立行政法人理化学研究所, 田中生体機能合成化学研究室, 准主任研究員 (00403098)
• Project Period (FY): 2011-04-01 – 2015-03-31
• Keywords: アザ電子環状反応 / 糖鎖 / 天然 / プローブ / タンパク質 / 蛍光 / イメージング / 糖タンパク質

Outline of Final Research Achievements

Tumor cell surface was fluorescently labeled by the investing’s innovative reaction under the quite mild conditions, and their metastatic potential in mice was efficiently visualized by noninvasive imaging. Especially, the tumor cells and their transfected versions expressing surface glycan-related genes were successfully imaged, and the glycan dependence on the metastatic potential was evaluated noninvasively. The cell surface method for introducing the various glycans and/or positron emitter for PET imaging has also been established. This investigator has succeeded in modifying selectively the specific molecules on the cell surface, thus the method developed in this research could be applicable to selectively introducing the functional glycans and/or labels in live systems, i.e., noninvasively imaging and regulation the tumor metastasis.

Free Research Field

有機合成化学、グライコケミカルバイオロジー、天然物化学、分子イメージング