2013 Fiscal Year Final Research Report
Single-molecule observation of a higher order structure of a proton channel in influenza virus
| Project/Area Number |
23685002
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
Physical chemistry
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| Research Institution | Tokyo Institute of Technology |
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Principal Investigator |
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Keywords | タンパク質 / １分子観察 / 低温 / 分光 / 構造 |
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| Research Abstract |
A laser-scanning confocal microscope was developed for three-dimensional (3D) imaging and co-localization of single biomolecules immobilized at a temperature of 1.5 K. The performance was demonstrated by taking 3-color 3D emission image of single quantum dots and 3D co-localization of single dots. The 3D image size of single dot with excitation wavelength of 635, 532, and 405 nm were all within 1.2 times of the diffraction-limited size. The 3D co-localization precision of single dot in images taken with 635 and 532 nm excitation was 3 nm in the lateral (x, y) and 18 nm in the axial (z) directions.
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