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2013 Fiscal Year Final Research Report

Detection and control of molecular orientation and reactivity of biomembrane model reaction field including proteins

Research Project

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Project/Area Number 23685003
Research Category

Grant-in-Aid for Young Scientists (A)

Allocation TypeSingle-year Grants
Research Field Physical chemistry
Research InstitutionToyohashi University of Technology

Principal Investigator

TERO Ryugo  豊橋技術科学大学, 工学(系)研究科(研究院), 准教授 (40390679)

Project Period (FY) 2011-04-01 – 2014-03-31
Keywords表面・界面 / 界面物理化学 / 脂質二重膜 / 原子間力顕微鏡 / 蛍光一分子観察
Research Abstract

I constructed a new biomembranes model system for the investigation of the active structures of lipid molecules which act as reactive factors of membrane proteins at cell membrane reactions. I fabricated a supported lipid bilayer (SLB) including phosphatidylinositol on mica substrate, and found that depression domains with 20 - 300 nm in lateral size and ~0.9 nm in depth in the PI-SLB. In-situ atomic force microscope (AFM) observation revealed that these submicron depression domains acted as the nucleation site of the self-assembly of F-BAR on the PI-SLB, a membrane deformation protein, and the surrounding region of the depression domains provided the F-BAR to the nucleation site. It is a novel result clearly showing the roles of submicron lipid domains during a protein reaction. I also established the fabrication methods of SLBs on graphene oxide and poly vinyl chloride membrane for the development of the methods to evaluate the molecular state and membrane properties in the SLBs.

  • Research Products

    (21 results)

All 2014 2013 2012 2011 Other

All Journal Article (7 results) (of which Peer Reviewed: 7 results) Presentation (7 results) (of which Invited: 7 results) Book (6 results) Remarks (1 results)

  • [Journal Article] Plasma irradiation to artificial cell membrane system at solid-liquid interface2014

    • Author(s)
      R. Tero, Y. Suda, R. Kato, H. Tanoue and H. Takikawa
    • Journal Title

      Appl. Phys. Express

      Volume: (in press)

    • URL

      http://iopscience.iop.org/1882-0786/

    • Peer Reviewed
  • [Journal Article] Formation and Fluidity Measurement of Supported Lipid Bilayer on Polyvinyl Chloride Membrane2014

    • Author(s)
      T. Kobayashi, A. Kono, M. Futagawa, K. Sawada and R. Tero
    • Journal Title

      AIP Conference Proceedings

      Volume: 1585 Pages: 145-152

    • DOI

      10.1063/1.4866633

    • Peer Reviewed
  • [Journal Article] Ecofriendly Route for the Synthesis of Highly Conductive Graphene Using Extremophiles for Green Electronics and Bioscience2013

    • Author(s)
      S. Raveendran, N. Chauhan, Y. Nakajima, H. Toshiaki, S. Kurosu, Y. Tanizawa, R. Tero, Y. Yoshida, T. Hanajiri, T. Maekawa, P. M. Ajayan, A. Sandhu and D. S. Kumar
    • Journal Title

      Part. Part. Syst. Charact

      Volume: 30 Pages: 573-578

    • DOI

      10.1002/ppsc.201200126

    • Peer Reviewed
  • [Journal Article] Substrate Effects on the Formation Process, Structure and Physicochemical Properties of Supported Lipid Bilayers2012

    • Author(s)
      R. Tero
    • Journal Title

      Materials

      Volume: 5 Pages: 2658-2680

    • DOI

      10.3390/ma5122658

    • Peer Reviewed
  • [Journal Article] Reduced Graphene Oxide as the Support for Lipid Bilayer Membrane2012

    • Author(s)
      K. Tsuzuki, Y. Okamoto, S. Iwasa, R. Ishikawa, A. Sandhu and R. Tero
    • Journal Title

      J. Phys.: Conf. Ser

      Volume: 352 Pages: 012016(7)

    • DOI

      10.1088/1742-6596/352/1/012016

    • Peer Reviewed
  • [Journal Article] Fabrication of Supported Lipid Bilayer on Graphene Oxide2012

    • Author(s)
      Y. Okamoto, K. Tsuzuki, S. Iwasa, R. Ishikawa, A. Sandhu and R. Tero
    • Journal Title

      J. Phys.: Conf. Ser

      Volume: 352 Pages: 012017(6)

    • DOI

      10.1088/1742-6596/352/1/012017

    • Peer Reviewed
  • [Journal Article] Anomalous Diffusion in Supported Lipid Bilayers Induced by Oxide Surface Nanostructures2011

    • Author(s)
      R. Tero, G. Sazaki, T. Ujihara and T. Urisu
    • Journal Title

      Langmuir

      Volume: 27 Pages: 9662-9665

    • DOI

      10.3390/ma5122658

    • Peer Reviewed
  • [Presentation] 支持平面脂質二重膜を用いた脂質とタンパク質の動的挙動のその場観察2013

    • Author(s)
      手老龍吾
    • Organizer
      第64回コロイドおよび界面化学討論会
    • Place of Presentation
      名古屋・名古屋工業大
    • Year and Date
      2013-09-20
    • Invited
  • [Presentation] 人工細胞膜中での脂質およびタンパク質の動的挙動の観察2013

    • Author(s)
      手老龍吾
    • Organizer
      第5回CIMoS(協奏分子システム研究センター)セミナー
    • Place of Presentation
      岡崎・分子研
    • Year and Date
      2013-09-10
    • Invited
  • [Presentation] グラフェン酸化物上への人工脂質膜プラットフォームの作製2013

    • Author(s)
      手老龍吾
    • Organizer
      第60回応用物理学会春季学術講演会
    • Place of Presentation
      厚木・神奈川工科大
    • Year and Date
      2013-03-27
    • Invited
  • [Presentation] Artificial Lipid Membranes on Graphene Oxide and Reduced Graphene Oxide2013

    • Author(s)
      R. Tero
    • Organizer
      CRC (Catalysis Research Center) International Symposium, New Challenges on the Bio-interface : Structures and Dynamics
    • Place of Presentation
      Sapporo, Japan
    • Year and Date
      2013-02-05
    • Invited
  • [Presentation] Single molecule/particle tracking in supported lipid bilayers on nanostructured substrates and graphene2012

    • Author(s)
      R. Tero
    • Organizer
      Engineering Lipid Bilayers 2012
    • Place of Presentation
      Leeds, UK
    • Year and Date
      2012-09-08
    • Invited
  • [Presentation] Supported lipid membranes on graphene oxide and reduced graphene oxide2012

    • Author(s)
      R. Tero
    • Organizer
      Crystal & Graphene Science Symposium - 2012
    • Place of Presentation
      Waltham, MA, USA
    • Year and Date
      2012-09-05
    • Invited
  • [Presentation] Tracking Single Molecular Diffusion at Oxide-Water Interfaces : Molecular Diffusion in Supported Lipid Bilayers on TiO_2(100)2011

    • Author(s)
      R. Tero, G. Sazaki, T. Ujihara and T. Urisu
    • Organizer
      International Symposium & The third Iwasawa Conference on Catalysis and Surface Sciences
    • Place of Presentation
      Xiamen, China
    • Year and Date
      2011-12-04
    • Invited
  • [Book] シャボン玉のように作れる細胞膜モデル,応用物理2014

    • Author(s)
      手老龍吾
    • Total Pages
      83, 136-139
    • Publisher
      応用物理学会
  • [Book] 脂質二重膜,問題と解説で学ぶ表面科学,日本表面科学会編2013

    • Author(s)
      手老龍吾
    • Total Pages
      127
    • Publisher
      共立出版
  • [Book] グラフェン酸化物上への細胞膜モデル系の作製とその観察, Molecular Electronics and Bioelectronics (応用物理学会有機分子・バイオエレクトロニクス分科会会誌)2013

    • Author(s)
      手老龍吾, 岡本吉晃, ムアザムシャー, 岩佐精二, サンドゥーアダルシュ
    • Total Pages
      24, 93-96
    • Publisher
      応用物理学会
  • [Book] 反応場としてのサポーティッドメンブレン,生物物理2012

    • Author(s)
      手老龍吾, 出羽毅久
    • Total Pages
      52, 283-286
    • Publisher
      日本生物物理学会
  • [Book] "Fabrication and Application of Plasmonic Silver Nanosheet", in Reviews in Plasmonics 2010, edited by C. D. Geddes2011

    • Author(s)
      K. Tamada, X. Li, P. Wulandari, T. Nagahiro, K. Michioka, M. Toma, K. Toma, D. Obara, T. Nakada, T. Hayashi, Y. Ikezoe, M. Hara, S. Katano, Y. Uehara, Y. Kimura, M. Niwano, R. Te ro and K. Okamoto
    • Total Pages
      139-157
    • Publisher
      Springer
  • [Book] 酸化物基板表面上の細胞膜モデルシステム:支持平面脂質膜のダイナミクスと基板表面の影響, Molecular Electronics and Bioelectronics (応用物理学会有機分子・バイオエレクトロニクス分科会会誌)2011

    • Author(s)
      手老龍吾, 佐崎元, 宇治原徹, 宇理須恒雄
    • Total Pages
      22, 89-92
    • Publisher
      応用物理学会
  • [Remarks] ホームページ等

    • URL

      http://www.eiiris.tut.ac.jp/tero/

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Published: 2015-07-16  

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