2013 Fiscal Year Final Research Report
Construction of virus genome libraries and exhaustive identification of viruses in water environment
| Project/Area Number |
23686075
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
Civil and environmental engineering
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| Research Institution | Tohoku University |
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Principal Investigator |
MASAGO Yoshifumi 東北大学, 未来科学技術共同研究センター, 准教授 (50507895)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Keywords | 病原ウイルス / 次世代シーケンシング / メタゲノム |
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| Research Abstract |
We investigated various viruses in water environment using next-generation sequencing (NGS) technology. Component technologies such as pre-treatment of environmental samples, molecular technologies for sequencing and bioinformatic analysis of obtained sequence data were examined and developed. In genotyping of noroviruses in wastewater, we could detect various genotypes including minor types such as GII.2. In metagenomic analysis of RNA viruses, we found that most viruses in wastewater are unidentified. We could detect viruses from 11 genera, some of which have not identified in Japan. These findings demonstrated that the developed methods for NGS analysis are very useful in identifying viruses in the environment.
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