Development of the generation process of multi-specific antibodies as next-generation therapeutic antibodies

2014 Fiscal Year Final Research Report

• Project/Area Number: 23686118
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Biofunction/Bioprocess
• Research Institution: Tohoku University
• Principal Investigator: ASANO Ryutaro 東北大学, 工学(系)研究科(研究院), 准教授 (80323103)
• Project Period (FY): 2011-04-01 – 2015-03-31
• Keywords: 癌 / 蛋白質 / バイオテクノロジー / 二特異性抗体 / ファージ提示法

Outline of Final Research Achievements

Here, we tried to develop the generation process of integrating the second specificity into a monoclonal antibody. Based on an anti-human epidermal growth factor receptor (EGFR) antibody, we examined a mutation site, mutant amino acid species, and evaluation method of binding activity. Using multiple libraries and living cell panning with mammalian cells artificially expressing target antigens, we finally succeeded in integrating another specificity into anti-EGFR antibody and evaluated them using enzyme-linked immunosorbent assay. We also confirmed general versatility of this process through developing another antibody with two specificities.

Free Research Field

応用生物工学