2014 Fiscal Year Final Research Report
Elucidation for the mitochondrial tRNA processing mechanism
| Project/Area Number |
23687015
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
Structural biochemistry
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| Research Institution | National Institute of Advanced Industrial Science and Technology |
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Principal Investigator |
NUMATA Tomoyuki 独立行政法人産業技術総合研究所, バイオメディカル研究部門, 主任研究員 (10401564)
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| Project Period (FY) |
2011-04-01 – 2015-03-31
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| Keywords | tRNA / アンチコドン / 修飾ヌクレオシド / tRNA修飾酵素 / 結晶構造解析 |
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| Outline of Final Research Achievements |
Post-transcriptional modifications at the tRNA anticodon loop are essential for precise decoding of the genetic code. The modification defects in mitochondrial tRNA are the main cause of the mitochondrial dysfunction. In this study, I determined the crystal structure of the tRNA modification enzyme, which is responsible for synthesizing the cyclic form of N6-threonylcarbamoyladenosine, as part of elucidating the tRNA modification mechanisms. Together with the structure-based mutational experiments, the amino acid residues crucial for the reaction were also identified.
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| Free Research Field |
構造生物化学
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