2013 Fiscal Year Final Research Report
Development of microfluidic mixer for time-resolved spectroscopy applicable to low-yield biomolecules
| Project/Area Number |
23687022
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
Biophysics
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| Research Institution | Institute of Physical and Chemical Research (2013)
Institute for Molecular Science (2011-2012) |
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Principal Investigator |
KIMURA Tetsunari 独立行政法人理化学研究所, 放射光科学総合研究センター, 研究員 (70506906)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Keywords | マイクロ流路 / フォールディング / 膜タンパク質 / フローセル / ストップト・フロー |
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| Research Abstract |
To understand the molecular mechanism of biomolecular reactions, the dynamics of the molecules along the reaction have to be revealed by the real-time measurements. However, the yield of protein is usually very low, ug-mg, and the time-resolved measurements is hard to apply for many biomolecular reactions because of the relatively large consumption. In this research, the time-resolved spectroscopies applicable to the low-yield protein samples were developed and applied to observe the folding, membrane transport and enzymatic reactions.
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[Journal Article] Formation of Host-Guest Complexes on Gold Surface Investigated by Surface-Enhanced IR Absorption Spectroscopy2014
Author(s)
Inokuchi, Y., Mizuuchi, T., Ebata, T., Ikeda, T., Haino, T., Kimura, T., Guo, H., Furutani, Y
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Journal Title
Chem. Phys. Lett
Volume: 592
Pages: 90-95
DOI
Peer Reviewed
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