Development of on-chip neuronal network measurement device using nanotechnology

2014 Fiscal Year Final Research Report

• Project/Area Number: 23687023
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Biophysics
• Research Institution: Tokyo Medical and Dental University (2013-2014); Kanagawa Academy of Science and Technology (2011-2012)
• Principal Investigator: TERAZONO Hideyuki 東京医科歯科大学, 生体材料工学研究所, 助教 (30398143)
• Project Period (FY): 2011-04-01 – 2015-03-31
• Keywords: センサアレイ / 神経ネットワーク / ナノ加工 / オンチップ / CMOS

Outline of Final Research Achievements

We developed a system using CMOS sensor array to detect extracellular action potential. Since CMOS sensor array consists of huge number of electrode, if cells directly contact sensor array, extracellular action potentials of neuronal network comprehensively can be detected. To detect action potential on the sensor array, we selected a sensor array of high-speed camera without photodiode array and micro lens array. As the result, coating with silicone polymer prevented the crush by culture medium and cells could be cultivated on the sensor array. Furthermore, sensor array filled with culture medium could detected electrical change generated with function generator. This result indicated the potential to detect the extracellular action potential.
Furthermore, we developed a new technique to adhere matured cells on the sensor array using calcium alginate gel. Using this technique, neurons could be detach from culture dish and recultivate without destructing synaptic connection.

Free Research Field

生物物理