Contribution of transcriptional corepressor ETO2 to the epigenome formation in erythroblasts

2012 Fiscal Year Final Research Report

• Project/Area Number: 23687024
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Molecular biology
• Research Institution: Tohoku University
• Principal Investigator: FUJIWARA Tohru 東北大学, 大学院・医学系研究科, 助教 (60333796)
• Project Period (FY): 2011 – 2012
• Keywords: 転写

Research Abstract

Overexpression of ETO2 as well as chromatin immunoprecipitation analysis in K562 human erythroid cell line revealed that ETO2 directly represses representative erythroid genes, including globin genes. Subsequently, we established erythroid differentiation system from cord blood CD34-positive cells. By using the system, we demonstrated that short hairpin RNA (shRNA)-mediated knockdown of ETO2 in cord blood CD34-positive cell-derived primary erythroblasts resulted in the significantupregulation of globin genes.

Research Products

• [Journal Article] Role of transcriptional corepressor ETO2 in erythroid cells (2013)
  • Author(s): Fujiwara T, Alqadi YW, Okitsu Y, Fukuhara N, Onishi Y, Ishizawa K, Harigae H
  • Journal Title: Experimental Hematology Volume: 41 Pages: 303-315
  • DOI: DOI:http://dx.doi.org/10.1016/j.exphem.2012.10.015
  • Peer Reviewed
• [Journal Article] Gene expression profiling identifies HOXB4 as a direct downstream target of GATA-2 in human CD34+ hematopoietic cells (2012)
  • Author(s): Fujiwara T, Yokoyama H, Okitsu Y, Kamata M, Fukuhara N, Onishi Y, Fujimaki S, Takahashi S, Ishizawa K, Bresnick EH, Harigae H
  • Journal Title: PLoS ONE Volume: 20127 Pages: e40959
  • DOI: DOI:http://dx.doi.org/10.1371/journal.pone.0040959
  • Peer Reviewed
• [Journal Article] Genome-wide analysis of GATA transcription factor in hematopoietic cells (2012)
  • Author(s): Fujiwara T
  • Journal Title: Rinsho Ketsueki Volume: 53 Pages: 287-294
  • DOI: DOI:http://dx.doi.org/10.11406/rinketsu.53.287
  • Peer Reviewed
• [Presentation] Regulation of erythropoiesis by histone methyltransferase EZH2 inhibitor 3-Deazaneplanocin A (DZNep) (2012)
  • Author(s): Fujiwara T, Saitoh H, Okitsu Y, Fukuhara N, Onishi Y, Ishizawa K, Ichinohasama R, Harigae H
  • Organizer: 米国血液学会
  • Place of Presentation: アト ランタ(米国)
  • Year and Date: 2012-12-08
• [Presentation] Exploring the mechanism of ETO2-dependent transcriptional regulation in erythroid cells (2011)
  • Author(s): Wael YA, Fujiwara T, Okitsu Y, Yasushi O, Ishizawa K, Harigae H
  • Organizer: 米国血液学会
  • Place of Presentation: サン ディエゴ(米国)
  • Year and Date: 2011-12-11
• [Presentation] Expression profiling ofETO2-regulated genes in erythroid cells (2011)
  • Author(s): Wael YA, Fujiwara T, Okitsu Y, Kohata K, Fukuhara N, Yasushi O, Yamamoto J, Ishizawa K, Harigae H
  • Organizer: 日本血液学会
  • Place of Presentation: 名古 屋
  • Year and Date: 2011-10-16
• [Presentation] Role of ETO2 in the epigenetic regulation of erythroid genes (2011)
  • Author(s): Fujiwara T, Wael YA, Harigae H
  • Organizer: 欧州血液学会
  • Place of Presentation: ロンドン(英国)
  • Year and Date: 2011-06-11
• [Remarks]
  • URL: http://www.rh.med.tohoku.ac.jp/