2013 Fiscal Year Final Research Report
Analysis of amino acid signaling in mammals and Drosophila
| Project/Area Number |
23687031
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
Cell biology
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| Research Institution | The Institute of Physical and Chemical Research |
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Principal Investigator |
NISHIMURA Takashi 独立行政法人理化学研究所, 発生・再生科学総合研究センター, チームリーダー (90568099)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Keywords | シグナル伝達 / アミノ酸 / 個体成長 / インスリン様成長因子 / ショウジョウバエ |
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| Research Abstract |
The multi-protein complex TORC1 is a key regulator of cell growth and size control in both Drosophila and mammals. To better understand amino acid signaling, we took a biochemical approach to identify the binding proteins of small GTPase RagA/C. We identified the glycolytic enzyme GAPDH and found that GAPDH likely regulates the TORC1 localization and activity through RagA/C.
In addition, to understand how Drosophila recognizes nutrition to control body growth, we focused on the molecular mechanism underlying the nutrient-dependent expression of Drosophila Insulin-like peptide (dilp). We identified the responsible transcription factors that cooperatively control dilp5 gene expression. We also conducted in vivo RNAi screening to identify novel players for the regulation of body growth. We identified a gene that we named SDR. SDR is a new class of secreted Dilp-binding proteins that negatively regulate the function of Dilps and thereby body growth.
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