Construction of an experimental model system that mimic the evolution of early life

2013 Fiscal Year Final Research Report

• Project/Area Number: 23687034
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Evolutionary biology
• Research Institution: Osaka University
• Principal Investigator: ICHIHASHI Norikazu 大阪大学, 情報科学研究科, 准教授 (20448096)
• Project Period (FY): 2011-04-01 – 2014-03-31
• Keywords: 進化 / 人工ゲノム / 実験進化 / RNAゲノム

Research Abstract

The evolutionary process from the assembly of chemical compounds to the present-day life is largely unknown. One of the major obstacles is the lack of the experimental model. In this project, we have succeeded in the construction of an experimental model that functionally mimic the early life and have the ability of autonomous evolution. This system would be useful for the experimental investigation of the evolutionary process from a simple genome replication system to a present-day complex life system.

Research Products

• [Journal Article] Effects of ribosomes on the kinetics of Qb replication (2014)
  • Author(s): Usui, K., Ichihashi, N., Kazuta, Y., Matsuura, T., Yomo, T
  • Journal Title: FEBS Letters Volume: 588 Pages: 117-123
  • DOI: 10.1016/j.febslet.2013.11.018
  • Peer Reviewed
• [Journal Article] Darwinian evolution in a translation-coupled RNA replication system within a cell-like compartment (2013)
  • Author(s): Ichihashi, N., Usui, K., Kazuta, Y., Sunami, T., Matsuura T., Yomo, T
  • Journal Title: Nature Communications Volume: 4 Pages: 1-7
  • DOI: 10.1038/ncomms3494
  • Peer Reviewed
• [Journal Article] Kinetic model of double-stranded RNA formation during long RNA replication by Qβreplicase (2013)
  • Author(s): Usui, K., Ichihashi, N., Kazuta, Y., Matsuura, T., Yomo, T
  • Journal Title: FEBS Letters Volume: 587 Pages: 2565-2571
  • DOI: 10.1016/j.febslet.2013.06.033
  • Peer Reviewed
• [Journal Article] A controllable gene expression system in liposomes that includes a positive feedback loop (2013)
  • Author(s): Kobori, S., Ichihashi, N., Kazuta, Y., Yomo, T
  • Journal Title: Molecular Biosystems Volume: 9 Pages: 1282 - 1285
  • DOI: 10.1261/rna.032748.112
  • Peer Reviewed
• [Journal Article] Effects of compartment size on the kinetics of intracompartmental multimeric orotein synthesis (2012)
  • Author(s): Matsuura, T., Hosoda, K., Kazuta, Y., Ichihashi, N., Suzuki, H., Yomo, T
  • Journal Title: ACS Synthetic Biology Volume: 1 Pages: 431−437
  • DOI: 10.1021/sb300041z
  • Peer Reviewed
• [Journal Article] α-Complementation in an artificial genome replication system in liposomes (2012)
  • Author(s): Nishiyama, K., Ichihashi, N., Matsuura, T., Kazuta, Y., Yomo, T
  • Journal Title: Chembiochem Volume: 13 Pages: 2701-2706
  • DOI: 10.1002/cbic.201200586
  • Peer Reviewed
• [Journal Article] Kinetic analysis of aptazyme-regulated gene expression in a cell-free translation system : modeling of ligand-dependent and -independent expression (2012)
  • Author(s): Kobori, S., Ichihashi, N., Kazuta, Y., Matsuura, T., Yomo, T
  • Journal Title: RNA Volume: 18 Pages: 1458-1465
  • DOI: 10.1261/rna.032748.112
  • Peer Reviewed
• [Journal Article] Importance of parasite RNA species repression for prolonged translation-coupled RNA self-replication (2012)
  • Author(s): Y Bansho, Y., Ichihashi, N., Kazuta, Y., Matsuura, T., Suzuki, H., Yomo, T
  • Journal Title: Chemistry and Biology Volume: 19 Pages: 478-487
  • DOI: 10.1016/j.chembiol.2012.01.019
  • Peer Reviewed
• [Presentation] The International Astrobiology Workshop 2013 (2013)
  • Author(s): Ichihashi, N
  • Organizer: as the Japan Astrobiology Network Annual Meeting #6
  • Place of Presentation: JAXA/ISAS,Sagamihara, Japan
  • Year and Date: 20131128-30
• [Presentation] The Thirteenth International Conference on the Synthesis and Simulation of Living Systems (2012)
  • Author(s): Ichihashi, N
  • Organizer: Artificial Life XIII
  • Place of Presentation: Michigan State University in East Lansing, Michigan, USA
  • Year and Date: 20120719–22
• [Book] Constructive approaches for the origin of life. A captor in"Genesis - in the Beginning : Precursors of Life, Chemical Models and Early Biological Evolution." (2012)
  • Author(s): Ichihashi, N., Matsuura, T., Kita, H., Sunami, T., Suzuki, H., Yomo, T(J. Seckbach Ed)
  • Total Pages: 289–304
  • Publisher: Springer
• [Remarks]
  • URL: http://www-symbio.ist.osaka-u.ac.jp/