Study on the mechanisms underlying biosynthesis and transport of salicylic acid using experimental systems in which salicylic acid is artificially inducible

2014 Fiscal Year Final Research Report

• Project/Area Number: 23688005
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Plant pathology
• Research Institution: Shinshu University
• Principal Investigator: KATOU Shinpei 信州大学, 学術研究院農学系, 准教授 (10533614)
• Research Collaborator: FUJIWARA Masayuki 奈良先端科学技術大学院大学, バイオサイエンス研究科, 助教 (70403350) ; ARAI Ryoichi 信州大学, 学術研究院繊維学系, 助教 (50344023)
• Project Period (FY): 2011-04-01 – 2015-03-31
• Keywords: 病害抵抗性 / サリチル酸 / 生合成機構 / 輸送機構 / 人為的誘導系

Outline of Final Research Achievements

Salicylic acid (SA) plays an important role in plant resistance to pathogens. Three main results of this study are as follows: 1) Peroxisomal β-oxidation and CBP60-type transfactor were identified as candidates for an intermediate of the SA biosynthesis pathway and its transcriptional regulator using an experimental system in which SA biosynthesis is artificially inducible. 2) Molecular mechanisms underlying high performance of AtICS1, known to be involved in SA synthesis in Arabidopsis thaliana, were clarified by comparative analyses with ICS homologs from other plant species. 3) An artificial SA synthase which shows high activity in plant leaves was produced by fusing bacterial enzymes that mediate SA synthesis.

Free Research Field

植物病理学