2013 Fiscal Year Final Research Report
Screening of novel fatty acid metabolism and development of the process for useful material production
| Project/Area Number |
23688011
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied microbiology
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| Research Institution | Kyoto University |
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Principal Investigator |
KISHINO Shigenobu 京都大学, (連合)農学研究科(研究院), 助教 (40432348)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Keywords | 応用微生物 / 脂質 / 酵素 / 発酵 / バイオテクノロジー |
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| Research Abstract |
I selected the microorganism which converted (E)-2-methyl-2-butenoic acid (tiglic acid, TGLA) to (S)-2-methylbutyric acid (S-2MBA). This reaction was found to be consisted of three reactions. Therefore, I screened the microorganisms to catalyze these reactions each other. Using selected microorganisms, S-2MBA production was improved.
I selected the microorganism which reduced the carboxyl group of C14 fatty acid to the corresponding alcohol, and from the more detailed analysis of this reaction, I was identified an enzyme that converted CoA form of C14 fatty acid to the corresponding aldehyde.
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