Systematic analysis of cross-regulatory profiles in two-component signal transduction systems among closely related bacterial pathogens

2014 Fiscal Year Final Research Report

• Project/Area Number: 23688013
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Applied biochemistry
• Research Institution: Kinki University
• Principal Investigator: KATO Akinori 近畿大学, 農学部, 講師 (00454645)
• Project Period (FY): 2011-04-01 – 2015-03-31
• Keywords: ネットワーク / シグナル伝達 / 進化 / 細菌 / 感染症 / two-component system / connector / ゲノム

Outline of Final Research Achievements

Two-component system (TCS) is a predominant mode of signal transduction in bacteria. Recent progresses have been drawing detailed pictures of their regulatory networks and evolutional traces. Yet, systematic experimental analyses across species and their statistics are quite challenging. Here, we aimed to analyze cross-regulatory profiles in TCSs of Salmonella enterica and its closely related bacteria by constructing approximately 30 TCS mutant strains for each species. In order to detect novel cross-regulatory phenomena in a quantitative manner, a representative gene promoter, which is directly regulated by a given TCS, was selected for each TCS and cloned into a promoter-less luciferase (lux) reporter/ broad range conjugation plasmid. By mating and testing epistasis in TCSs, we compare extended “TCSs cross-regulatory profiling maps” across species in details. From a certain aspect of these relating analyses, novel TCS regulatory mechanisms were established lately.

Free Research Field

分子遺伝学