2012 Fiscal Year Final Research Report
Mechanisms of colon cancer stem cell self-renewal and pluripotency
| Project/Area Number |
23689019
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
Pathological medical chemistry
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| Research Institution | The University of Tokyo |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2012
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| Keywords | 癌幹細胞 / 大腸癌 |
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| Research Abstract |
Cancer stem cells are thought to be responsible for both metastasis and cancer recurrence. We utilized FACS sorting to isolate cancer stem cells (CSCs) and non-cancer stem cells from xenograft human colon tumors to analyze gene expression. RNA-seq analysis revealed that 374 genes were significantly up-regulated in CSCs. Furthermore, we found that knockdown of any of 13 genes resulted in decreased growth ofCSCs. Hereafter, we will investigate the importance of these genes in CSC behavior.
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