Roles of the ubiquitin ligase, SCRAPPER, in intracellular transport

2014 Fiscal Year Final Research Report

• Project/Area Number: 23689021
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Pathological medical chemistry
• Research Institution: Hamamatsu University School of Medicine (2013); Kansai Medical University (2011-2012)
• Principal Investigator: YAO Ikuko 浜松医科大学, メディカルフォトニクス研究センター, 准教授 (60399681)
• Project Period (FY): 2011-04-01 – 2015-03-31
• Keywords: シナプス / 蛋白質 / 神経可塑性 / ユビキチン / SCRAPPER / 質量分析 / イメージング / 老化

Outline of Final Research Achievements

Selective protein degradation is crucial in regulation of various cellular functions. The degradation machinery is supposed to play important roles in intracellular transport. To address this issue, we studied the regulation of the dynamics of motor protein by the ubiquitin ligase, especially focused on SCRAPPER, which we previously identified (Yao et al., Cell 2007)
We revealed reciprocal interactions between SCRAPPER and the motor protein and found that the amount of the motor protein was regulated by SCRAPPER dependent ubiquitination and the proteasomal degradation. With mass spectrometry imaging technique, we also identified some candidates of molecules that showed significant modulation in the brain sections of Scrapper gene knockout mice compared with those of wild type mice.

Free Research Field

神経科学、生化学、イメージング