Study on mechanisms for formation of genome replication vesicles produced by plus-strand RNA viruses

2013 Fiscal Year Final Research Report

• Project/Area Number: 23689028
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Virology
• Research Institution: National Institute of Infectious Diseases
• Principal Investigator: MORI Yoshio 国立感染症研究所, その他部局等, 室長 (40379095)
• Project Period (FY): 2011-04-01 – 2014-03-31
• Keywords: ウイルス / 細胞

Research Abstract

The common hallmark of plus-strand RNA viruses is to synthesize their genomes at various organelle membranes. In this study, we attempted to isolate viral and host factors for genome synthesis of plus-strand RNA viruses and common mechanisms underlying genome replication. In order to quantify, the subgenomic replicon of rubella virus, a member of plus-strand RNA viruses, was established in this study, and using this system viral and host factors for promoting viral propagation were identified. Furthermore, this is the first study to indicate that rubella virus undergoes the genome synthesis at the plasma membrane. The results in this study provide the information for understanding of mechanisms underlying genome replication of plus-strand RNA viruses.

Research Products

• [Journal Article] Japanese encephalitis virus core protein inhibits stress granule formation through an interaction with Caprin-1 and facilitates viral propagation (2013)
  • Author(s): Katoh H, Okamoto T, Fukuhara T, Kambara H, Morita E, Mori Y, Kamitani W, Matsuura Y
  • Journal Title: J.Virol Volume: 87 Pages: 489-502
• [Journal Article] Immuno-electron microscopy of primary cell cultures from genetically modified animals in liquid by atmospheric scanning electron microscopy (2013)
  • Author(s): Kinoshita T, Mori Y, Hirano K, Sugimoto S, Okuda K, Matsumoto S, Namiki, Ebihara R, Kawata M, Nishiyama H, Sato M, Suga M, Higashiyama K, Sonomoto S, Mizunoe Y, Nishihara S, Sato C
  • Journal Title: Microscopy and microanalysis Volume: 20 Pages: 469-483
• [Journal Article] Nationwide Rubella (2013)
  • Author(s): Tanaka-Taya K, Sato H, Arai S, Yamagishi T Yahata Y, Nakayama K, Sugawara T Ohkusa Y, Matsui T, Saito T, Kanou K, Shimada T, Kinoshita H, Yamashita K, Yasui Y, Tada Y, Mori Y, Takeda M, Sunagawa T, Oishi K
  • Journal Title: Epidemic-Japan Volume: 62(27) Pages: 457-462
• [Journal Article] Heterogeneous nuclear ribonucleoprotein A2 participates in the replication of Japanese encephalitis virus through an interaction with viral proteins and RNA (2011)
  • Author(s): Katoh H, Mori Y, Kambara H, Abe T, Fukuhara T, Morita E, Moriishi K, Kamitani W, Matsuura Y
  • Journal Title: J.Virol Volume: 85(21) Pages: 10976-10988
• [Journal Article] Involvement of cyclophilin B in the replication of Japanese encephalitis virus (2011)
  • Author(s): Kambara H, Tani H, Mori Y, Abe T, Katoh H, Fukuhara T, Taguwa S, Moriishi K, Matsuura Y
  • Journal Title: Virology Volume: 412(1) Pages: 211-219, 2011
• [Journal Article] Elucidation of the Full Genetic Information of Japanese Rubella Vaccines and the Genetic Changes Associated with In Vitro and In Vivo Vaccine Virus Phenotypes (2011)
  • Author(s): Otsuki N, Abo H, Kubota T, Mori Y, Umino Y, Okamoto K, Takeda M, Komase K
  • Journal Title: Vaccine Volume: 29(10) Pages: 1863-1873
• [Presentation] (2013)
  • Author(s): Mori Y
  • Organizer: The 7^<th> China-Korea-Japan forum on communicable disease control and prevention
  • Year and Date: 20131100
• [Presentation] Molecular epidemiology and laboratory diagnosis of rubella virus in Japan (2013)
  • Author(s): Mori Y
  • Organizer: The 10^<th> Japan-Taiwan symposium on vaccine preventable diseases and vector-borne diseases
  • Year and Date: 20130900
• [Presentation] 風疹ウイルスCタンパク質とp150タンパク質の共局在がウイルス産生に与える影響 (2012)
  • Author(s): 坂田真史、岡本貴世子、大槻紀之、安楽正輝、竹田誠、森嘉生
  • Organizer: 第60回日本ウイルス学会学術集会
  • Year and Date: 20121100
• [Presentation] Rubella virus undergoes genome synthesis at the plasma membrane with induction of filopodia (2012)
  • Author(s): Mori Y, Okamoto K, Sakata M, Otsuki N, Takeda M
  • Organizer: 34^<th> Naito conference
  • Year and Date: 20121000
• [Presentation] N-terminal hydrophobic amino acid residues of the capsid protein are critical for co-localization with the p150 protein and production of rubella virus (2012)
  • Author(s): Sakata M, Okamoto K, Otsuki N, Anraku M, Takeda M, Mori Y
  • Organizer: 11^<th> Awaji International forum on infection and immunity
  • Year and Date: 20120900
• [Presentation] The plasma membrane is the genome replication site for rubella virus (2011)
  • Author(s): Mori Y, Okamoto K, Sakata M, Otsuki N, Abo H, Takeda M
  • Organizer: XV International Congress of Virology
  • Year and Date: 20110900
• [Presentation] The short N-terminal region of the rubella virus capsid protein is critical to co-localize with the nonstructural p150 protein (2011)
  • Author(s): Sakata M, Okamoto K, Otsuki N, Abo H, Takeda M, Mori Y
  • Organizer: XV International Congress of Virology
  • Year and Date: 20110900
• [Presentation] Molecular mechanisms of the temperature-sensitive phenotype of live attenuated Japanese rubella vaccines (2011)
  • Author(s): Otsuki N, Sakata M, Okamoto K, Abo H, Kano K, Komase K, Takeda M, Mori Y
  • Organizer: XV International Congress of Virology
  • Year and Date: 20110900
• [Presentation] Heterogeneous nuclear ribonucleoprotein A2 participates in the replication of Japanese encephalitis virus through an interaction with viral proteins and RNA (2011)
  • Author(s): Katoh H, Mori Y, Kambara H, Kamitani W, Matsuura Y
  • Organizer: XV International Congress of Virology
  • Year and Date: 20110900
• [Presentation] Involvement of cyclophilin B in the replication of Japanese encephalitis virus (2011)
  • Author(s): Kambara H, Tani H, Mori Y, Abe T, Katoh H, Fukuhara T, Taguwa S, Moriishi K, Matsuura Y
  • Organizer: XV International Congress of Virology
  • Year and Date: 20110900