2013 Fiscal Year Final Research Report
Develop the method to measure the autophagic activity
| Project/Area Number |
23689032
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
Laboratory medicine
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| Research Institution | Niigata University (2012-2013)
Kyushu University (2011) |
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Principal Investigator |
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Keywords | 臨床検査医学 / オートファジー |
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| Research Abstract |
Autophagy is a catabolic process that degrades cytoplasmic components and organelles, and is maintain cellular homeostasis by eliminating unnecessary cellular components. Thus, the reduction of autophagic activity relates several disorders. However, it is difficult to measure the autophagic activity of patients. The goal of this study is to establish the method to measure the autophagic activity of patients for clinical use. As an initial step, we tried to observe autophagy in cultured mammalian cells. To observe autophagy, we established pH-sensitive fluorescent protein Keima expressing cell lines (Keima cells). Using Keima cells, we succeeded to observe autophagy sensitively and semi-quantitatively.
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Research Products
(17 results)
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[Journal Article] Casein kinase 2 is essential for mitophagy2013
Author(s)
Kanki T, Kurihara Y, Jin X, Goda T, Ono Y, Aihara M, Hirota Y, Saigusa T, Aoki Y, Uchiumi T, Kang D
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Journal Title
EMBO Rep
Volume: 14(9)
Pages: 788-94
DOI
Peer Reviewed
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[Journal Article] Protein instability and functional defect by mutations of dihydroorotate dehydrogenase with Miller syndrome patients2012
Author(s)
Fang J, Uchiumi T, Yagi M, Matsumoto S, Amamoto R, Saito T, Takazaki S, Kanki T, Yamaza H, Nonaka K, Kang D
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Journal Title
Biosci Rep
Volume: 32(6)
Pages: 631-9
DOI
Peer Reviewed
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