2013 Fiscal Year Final Research Report
Epigenetic regulation of liver cancer stem cells and establishment of a novel therapeutic approach
Project/Area Number |
23689039
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Research Category |
Grant-in-Aid for Young Scientists (A)
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Allocation Type | Single-year Grants |
Research Field |
Gastroenterology
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Research Institution | Chiba University |
Principal Investigator |
CHIBA Tetsuhiro 千葉大学, 医学(系)研究科(研究院), 講師 (00381583)
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Project Period (FY) |
2011-04-01 – 2013-03-31
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Keywords | エピジェネティクス / 肝癌 / 肝臓 / 幹細胞 |
Research Abstract |
Polycomb group protein EZH2 is essential for the self-renewal of liver cancer stem cells. Short hairpin-RNA against EZH2 and 3-Deazaneplanocin A (DZNep) promotes the conversion toward differentiated progeny cells and reduced tumorigenic ability thorough the inhibition of H3K27 trimethylation (H3K27me3). We also conducted chromatin immunoprecipitation sequencing (ChIP-seq) analysis of normal hepatic stem cells using anti-H3K27me3 and anti-H3K4me3 antibodies, and successfully identified 562 bivalent genes. In combination with microarray data in the differentiation induction, candidate genes closely associated with hepatocytes and/or cholangiocytes were selected from bivalent genes. Since cancer-related genes were included among these genes, further analyses would be necessary to examine the role of these molecules in normal hepatic stem cells and liver cancer stem cells.
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[Journal Article] Disulfiram eradicates tumor-initiating hepatocellular carcinoma cells in ROS-p38 MAPK pathway-dependent and -independent manners2014
Author(s)
Chiba T, Suzuki E, Yuki K, Zen Y, Oshima M, Miyagi S, Saraya A, Koide S, Motoyama T, Ogasawara S, Ooka Y, Tawada A, Nakatsura T, Hayashi T, Yamashita T, Kaneko S, Miyazaki M, Iwama A, Yokosuka O
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Journal Title
PLoS One
Volume: 9(1)
Pages: e84807
DOI
Peer Reviewed
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[Journal Article] The TIF1β-HP1 System Maintains Transcriptional Integrity of Hematopoietic Stem Cells2014
Author(s)
Miyagi S, Koide S, Saraya A, Wendt GR, Oshima M, Konuma T, Yamazaki S, Mochizuki-Kashio M, Nakajima-Takagi Y, Wang C, Chiba T, Kitabayashi I, Nakauchi H, Iwama A
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Journal Title
Stem Cell Reports
Volume: 23;2(2)
Pages: 145-52
DOI
Peer Reviewed
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[Journal Article] Effect of metformin on residual cells after chemotherapy in a human lung adenocarcinoma cell line2013
Author(s)
Kitazono S, Takiguchi Y, Ashinuma H, Saito-Kitazono M, Kitamura A, Chiba T, Sakaida E, Sekine I, Tada Y, Kurosu K, Sakao S, Tanabe N, Iwama A, Yokosuka O, Tatsumi K
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Journal Title
Int J Oncol
Volume: 43(6)
Pages: 1846-54
DOI
Peer Reviewed
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[Journal Article] Efficacy of transcatheter arterial chemoembolization with miriplatin-lipiodol water-soluble contrast agent emulsion in patients with hepatocellular carcinoma2013
Author(s)
Okimoto K, Ogasawara S, Chiba T, Ooka Y, Oobu M, Azemoto R, Kanogawa N, Motoyama T, Suzuki E, Tawada A, Yoshikawa M, Yokosuka O
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Journal Title
Anticancer Res
Volume: 33(12)
Pages: 5603-9
Peer Reviewed
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[Journal Article] Metformin, a diabetes drug, eliminates tumor-initiating hepatocellular carcinoma cells2013
Author(s)
Saito T, Chiba T, Yuki K, Zen Y, Oshima M, Koide S, Motoyama T, Ogasawara S, Suzuki E, Ooka Y, Tawada A, Tada M, Kanai F, Takiguchi Y, Iwama A, Yokosuka O
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Journal Title
PLoS One
Volume: 29;8(7)
Pages: e70010
DOI
Peer Reviewed
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[Journal Article] Adherence to medication is a more important contributor to viral breakthrough in chronic hepatitis B patients treated with entecavir than in those with Lamivudine2013
Author(s)
Kamezaki H, Kanda T, Arai M, Wu S, Nakamoto S, Chiba T, Maruyama H, Fujiwara K, Kanai F, Imazeki F, Nomura F, Yokosuka O
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Journal Title
Int J Med Sci
Volume: 10(5)
Pages: 567-74
DOI
Peer Reviewed
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[Journal Article] Ezh2 augments leukemogenicity by reinforcing differentiation blockage in acute myeloid leukemia2012
Author(s)
Tanaka S, Miyagi S, Sashida G, Chiba T, Yuan J, Mochizuki-Kashio M, Suzuki Y, Sugano S, Nakaseko C, Yokote K, Koseki H, Iwama A
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Journal Title
Blood
Volume: 2;120(5)
Pages: 1107-17
DOI
Peer Reviewed
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[Journal Article] Antiproliferative action of metformin in human lung cancer cell lines2012
Author(s)
Ashinuma H, Takiguchi Y, Kitazono S, Kitazono-Saitoh M, Kitamura A, Chiba T, Tada Y, Kurosu K, Sakaida E, Sekine I, Tanabe N, Iwama A, Yokosuka O, Tatsumi K
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Journal Title
Oncol Rep
Volume: 28(1)
Pages: 8-14
DOI
Peer Reviewed
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[Journal Article] 3-Deazaneplanocin A is a promising therapeutic agent for the eradication of tumor-initiating hepatocellular carcinoma cells2012
Author(s)
Chiba T, Suzuki E, Negishi M, Saraya A, Miyagi S, Konuma T, Tanaka S, Tada M, Kanai F, Imazeki F, Iwama A, Yokosuka O
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Journal Title
Int J Cancer
Volume: 1;130(11)
Pages: 2557-67
DOI
Peer Reviewed
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