2013 Fiscal Year Final Research Report
Basic approach for investigating epigenetic therapeutic strategies for bone and joint diseases
| Project/Area Number |
23689066
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Ehime University (2012-2013)
The University of Tokyo (2011) |
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Principal Investigator |
IMAI YUUKI 愛媛大学, プロテオサイエンスセンター, 教授 (10423873)
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| Project Period (FY) |
2011-11-18 – 2014-03-31
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| Keywords | 骨関節疾患 / エピゲノム / 軟骨細胞 / 破骨細胞 / クロマチン |
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| Research Abstract |
To clarify regulatory mechanism on epigenetics in bone and joint diseases, we identified Aire as a novel regulator in chondrocyte differentiation using gene expression microarray. Aire possesses PHD domain, which recognizes hypomethylated lysine residues of histone proteins. As the results of analyses of molecular function, Aire can facilitate chondrocyte differentiation through regulating status of histone H3K4me2 in promoter region of Bmp-2 (Si et al BBRC 2013).
In terms of osteoclasts, which resorb bone matrix, we tried to figure out dynamic chromatin remodeling during osteoclastogenesis using DNase-seq. Based on the combined information from gene expression and DNase-seq, we successfully identified novel transcription factors in osteoclastogenesis (Inoue et al. JBMR 2014 in press).
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[Journal Article] Estrogen receptorαin osteocytes regulates trabecular bone formation in female mice2014
Author(s)
Kondoh S, Inoue K, Igrashi K, Sugizaki H, Shirode-Fukuda Y, Inoue E, Yu T, Takeuchi J, Kanno J, Bonewald L, Imai Y
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Journal Title
Bone
Volume: 60
Pages: 68-77
DOI
Peer Reviewed
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[Journal Article] Vitamin D receptor in osteoblasts is a negative regulator of bone mass control2013
Author(s)
Yamamoto Y, Yoshizawa T, Fukuda T, Shirode-Fukuda Y, Yu T, Sekine K, Sato T, Kawano H, Aihara K, Nakamichi Y, Watanabe T, Shindo M, Inoue K, Inoue E, Tsuji N, Hoshino M, Karsenty G, Metzger D, Chambon P, Kato S, Imai Y
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Journal Title
Endocrinology
Volume: 154(3)
Pages: 1008-20
DOI
Peer Reviewed
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[Journal Article] JMJD5, a JmjC-domain-containing protein, negatively regulates osteoclastogenesis by facilitating NFATc1 protein degradation2012
Author(s)
Youn MY, Yokoyama A, Fujiyama-Nakamura S, Ohtake F, Minehata KI, Yasuda H, Suzuki T, Kato S, Imai Y
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Journal Title
J Biol Chem
Volume: 287(16)
Pages: 12994-3004
DOI
Peer Reviewed
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[Journal Article] GlcNAcylation of histone H2B facilitates its monoubiquitination2011
Author(s)
Fujiki R, Hashiba W, Sekine H, Yokoyama A, Chikanishi T, Ito S, Imai Y, Kim JH, He HH, Igarashi K, Kanno J, Ohtake F, Kitagawa H, Roeder R, Brown M, Kato S
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Journal Title
Nature
Volume: 480(7378)
Pages: 557-60
DOI
Peer Reviewed
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[Journal Article] PKA-dependent regulation of the histone lysine demethylase complex PHF2-ARID5B2011
Author(s)
Baba A, Ohtake F, Okuno Y, Yokota K, Okada M, Imai Y, Ni M, Meyer CA, Igarashi K, Kanno J, Brown M, Kato S
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Journal Title
Nat Cell Biol
Volume: 13(6)
Pages: 668-75
DOI
Peer Reviewed
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