Establishment of periodontal tissue regeneration method by using of novel PDLSC cell surface marker and PDL marker

2013 Fiscal Year Final Research Report

• Project/Area Number: 23689077
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Conservative dentistry
• Research Institution: Kyushu University
• Principal Investigator: WADA Naohisa 九州大学, 大学病院, 講師 (60380466)
• Project Period (FY): 2011-04-01 – 2014-03-31
• Keywords: 歯根膜組織 / 間葉系幹細胞 / 再生

Research Abstract

We found that Semaphorin 3A (Sema3A) can induce mesenchymal-stem-like properies in human periodontal ligament (PDL) cells, and Wnt5a can induce collagen fibrillogenesis which is main component of PDL tissue, by human PDL cells (HPDLC). Sema3A-overexpressing HPDLC exhibited an enhanced capacity to differentiate into both functional osteoblasts and adipocytes, and upregulated the expression of stem cell markers. Meanwhile, Wnt5a suppressed osteoblastic differentiation of HPDLC, while it significantly enhanced proliferation and migration of HPDLC, and PDL-related gene expression and collagen production in HPDLC. Both knockdown of periostin gene expression and inhibition of TGF-beta1 function suppressed the Wnt5a-induced PDL-related gene expression and collagen production. Although further studies are required to clarify the precise functional mechanism of both factors, Sema3A and Wnt5a can be factors with potential to regenerate PDL tissue efficiently.

Research Products

• [Journal Article] Semaphorin 3A induces mesenchymal stem-like properties in human periodontal ligament cells (2014)
  • Author(s): Wada N, Maeda H, Hasegawa D, Gronthos S, Bartold PM, Menicanin D, Fujii S, Yoshida S, Tomokiyo A, Monnouchi S, Akamine A
  • Journal Title: Stem Cells Dev Volume: (Epub ahead of print)
  • DOI: 10.1089/scd.2013.0405
• [Journal Article] Periodontal Ligament Derived Stem Cells Exhibit the Capacity for Long-Term Survival, Self-Renewal and Regeneration of Multiple Tissue Types in Vivo (2014)
  • Author(s): Menicanin D, Mrozik M, Wada N, Marino V, Shi S, Bartold PM, Gronthos S
  • Journal Title: Stem Cells Dev Volume: 23(9) Pages: 1001-1011
  • DOI: 10.1089/scd.2013.0490
• [Journal Article] Regeneration of the periodontium for preservation of the damaged tooth (2014)
  • Author(s): Maeda H, Tomokiyo A, Wada N, Koori K, Kawachi G, Akamine A
  • Journal Title: Histol Histopathol Volume: (Epub ahead of print)
  • URL: http://www.hh.um.es/Reviews-proofs/11-489-manuscript.pdf
• [Journal Article] Immunomodulatory Effects of Stem Cells (2013)
  • Author(s): Wada N, Gronthos S, Bartold PM
  • Journal Title: Periodontol 2000 Volume: 63(1) Pages: 198-216
  • DOI: 10.1111/prd.12024
• [Journal Article] Prospective Potency of TGF-β1 on Maintenance and Regeneration of Periodontal Tissue (2013)
  • Author(s): Maeda H, Wada N, Tomokiyo A, Monnouchi S, Akamine A
  • Journal Title: Int Rev Cell Mol Biol Volume: 304 Pages: 283-367
  • DOI: 10.1016/B978-0-12-407696-9.00006-3
• [Journal Article] Promise of periodontal ligament stem cells in regeneration of periodontium (2011)
  • Author(s): Maeda H, Tomokiyo A, Fujii S, Wada N, Akamine A
  • Journal Title: Stem Cell Res Ther Volume: 2 Pages: 33
  • DOI: 10.1186/scrt74
• [Presentation] Wnt5aはTGFβ1を介してヒト歯根膜細胞のコラーゲン線維形成を促進する (2014)
  • Author(s): 長谷川大学、和田尚久、前田英史、吉田晋一郎、門野内聡、御手洗裕美、濱野さゆり、祐田明香、赤峰昭文
  • Organizer: 第140回日本歯科保存学会春季学術大会
  • Place of Presentation: 滋賀県立芸術劇場(大津市)
  • Year and Date: 20140619-20
• [Presentation] The Effects of Wnt5a on Human Periodontal Ligament Cells (2014)
  • Author(s): Hasegawa D, Wada N, Maeda H, Yoshida S, Monnouchi S, Koori K, Hamano S, Mitarai H, Akamine A
  • Organizer: Kyudai Oral Bioscience 2014 - 8th International Symposium -
  • Place of Presentation: Fukuoka Recent Hotel, Fukuoka, Japan
  • Year and Date: 20140228-0301
• [Presentation] Wnt5aがヒト歯根膜細胞に及ぼす影響について (2013)
  • Author(s): 長谷川大学、和田尚久、前田英史、友清淳、門野内聡、郡勝明、吉田晋一郎、寺松陽子、濱野さゆり、祐田明香、杉井英樹、赤峰昭文
  • Organizer: 第139回日本歯科保存学会秋季学術大会
  • Place of Presentation: 秋田県総合生活文化会館(秋田市)
  • Year and Date: 20131017-18
• [Presentation] Conversion of periodontal ligament cells into multipotent stem-like cells (2013)
  • Author(s): Wada N, Maeda H, Hasegawa D, Gronthos S, Bartold PM, Menicanin D, Fujii S, Wada H, Tomokiyo A, Monnouchi S, Akamine A
  • Organizer: 91st General Session & Exhibition of the IADR
  • Place of Presentation: Seattle, Washington
  • Year and Date: 20130320-23
• [Presentation] 再生医療における歯原性幹細胞の可能性 (2013)
  • Author(s): 和田尚久
  • Organizer: 日本再生歯科医学会2013シンポジウム「よみがえる歯髄・歯根膜・根尖歯周組織」
  • Place of Presentation: 西日本総合展示場AIMビル(北九州市)
  • Year and Date: 2013-02-16
• [Presentation] Induction of stem/undifferentiated cells from human periodontal ligament cells by Sema3A (2012)
  • Author(s): Wada N, Maeda H, Hasegawa D, Gronthos S, Bartold PM, Menicanin D, Fujii S, Wada H, Tomokiyo A, Monnouchi S, Akamine A
  • Organizer: The Australian Health and Medical Research Congress 2012
  • Place of Presentation: Adelaide Convention Centre, Adelaide, Australia
  • Year and Date: 20121125-28
• [Presentation] 分化能の異なるヒト歯根膜細胞クローンの単離及びキャラクタライゼーション (2012)
  • Author(s): 長谷川大学、和田尚久、前田英史、藤井慎介、郡勝明、友清淳、門野内聡、河野清美、山本直秀、寺松陽子、濱野さゆり、祐田明香、赤峰昭文
  • Organizer: 第137回日本歯科保存学会秋季学術大会
  • Place of Presentation: 広島国際会議場(広島市)
  • Year and Date: 20121122-23
• [Book] Differentiation of Periodontal Ligament Stem /Progenitor Cells : Roles of TGF-β1. In : Stem Cells and Cancer Stem Cells, volume 4, Therapeutic Applications in Disease and Injury. Hayat MA (ed)(Chapter 5) (2012)
  • Author(s): Maeda H, Fujii S, Monnouchi S, Wada N, Akamine A
  • Total Pages: 51-58
  • Publisher: Springer, Heidelberg, Germany
• [Book] Periodontal ligament stem cells. In : Stem Cells in Clinic and Research. Gholamrezanezhad A (ed)(Chapter 25) (2011)
  • Author(s): Maeda H, Wada N, Fujii S, Tomokiyo A, Akamine A
  • Total Pages: 619-636
  • Publisher: InTech, Rijeka, Croatia