Identification of osteogenic transcriptional networks by chromatin immunoprecipitation sequencing and genetic approaches

2014 Fiscal Year Final Research Report

• Project/Area Number: 23689079
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Surgical dentistry
• Research Institution: The University of Tokyo
• Principal Investigator: OHBA Shinsuke 東京大学, 工学(系)研究科(研究院), 准教授 (20466733)
• Project Period (FY): 2011-04-01 – 2015-03-31
• Keywords: 骨芽細胞 / 転写

Outline of Final Research Achievements

This study aims to determine molecular mechanisms through which transcriptional regulators that are required for skeletal formation regulate differentiation of skeletal progenitors. Chromatin immunoprecipitation sequencing (ChIP-seq) and expression profiling in mouse primary osteoblasts and chondrocytes identified binding regions and target genes of the regulators in a genome-wide manner. Bioinformatic analyses of the data set provided genome-wide evidences for modes of action of each factor and its biological significance. These findings may help us understand mechanisms underlying the regulation of skeletal formation and lead to basic knowledge to achieve skeletal regenerative medicine.

Free Research Field

骨軟骨生物学