2013 Fiscal Year Final Research Report
Analysis of robustness of metabolic networks focusing on environmental dependency of gene knockouts
| Project/Area Number |
23700353
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Bioinformatics/Life informatics
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| Research Institution | Institute of Physical and Chemical Research (2013)
Ritsumeikan University (2011-2012) |
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Principal Investigator |
TOHSATO Yukako 独立行政法人理化学研究所, 生命システム研究センター, 研究員 (80346171)
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| Project Period (FY) |
2011 – 2013
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| Keywords | バイオインフォマティクス / マイクロアレイ / 表現型 / 数理モデル / 統計解析 |
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| Research Abstract |
Systematic studies of genotype-phenotype correlation have revealed that most single- knockout mutants often show no observable phenotype during growth under laboratory conditions. However, such studies have mainly examined phenotypes under a limited number of conditions such as Glucose and LB mediums. Genes showing no phenotypes when absent under one condition may exhibit phenotypic changes under different specific conditions, if the gene function depends on environmental conditions. To elucidate the environmental dependency of genes, we analyzed growth data of wild-type and single-knockout mutant in Escherichia coli in 1920 medium conditions obtained from the Phenotype MicroArray. Furthermore, we develop a kinetic model for the central metabolism of E. coli that includes not only the glucose transport system, but also the glycerol transport system.
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