Functional roles of IQ-ArfGEF at excitatory postsynaptic sites

2012 Fiscal Year Final Research Report

• Project/Area Number: 23700416
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Nerve anatomy/Neuropathology
• Research Institution: Kitasato University
• Principal Investigator: FUKAYA Masahiro 北里大学, 医学部, 講師 (10360900)
• Project Period (FY): 2011 – 2012
• Keywords: IQ-ArfGEF / 精神遅滞 / ノックアウトマウス / シナプス

Research Abstract

In this study, I focused on IQ-ArfGEF, one of the excitatory postsynaptic molecules, which causes nonsyndromic mental retardation. To reveal the functional mechanisms of IQ-ArfGEF at postsynaptic sites, IQ-ArfGEF knockout mice were generated. Using IQ-ArfGEF knockout mice, many neuronal and glial marker molecules were screened by immunoblot and immunohistochemical assays. Consequently, impairment of IQ-ArfGEF was accompanied by upregulation of some neural molecules in the brain.

Research Products

• [Journal Article] Degeneration and impaired regeneration of gray matter oligodendrocytes in amyotrophic lateral sclerosis (2013)
  • Author(s): Kang S, Li Y, Fukaya M, Lorenzini I, Cleveland DW, Ostrow LW, Rothenstein JD, Bergles DE
  • Journal Title: Nat Neurosci Volume: 16(5) Pages: 571-579
  • DOI: DOI:10.1038/nn.3357
  • Peer Reviewed
• [Journal Article] Distinct synaptic localization patterns of brefeldin A-resistant guanine nucleotide exchange factors BRAG2 and BRAG3 in the mouse retina (2013)
  • Author(s): Sakagami H, Katsumata O, Hara Y, Tamaki H, Watanabe M, Harvey RJ, Fukaya M
  • Journal Title: J Comp Neurol Volume: 521(4) Pages: 860-876
  • DOI: DOI:10.1002/cne.23206
  • Peer Reviewed
• [Journal Article] Accumulation of alpha-synuclein triggered by presynaptic dysfunction (2012)
  • Author(s): Nakata Y, Yasuda T, Fukaya M, Yamamori S, Itakura M, Nihira T, Hayakawa H, Kawanami A, Kataoka M, Nagai M, Sakagami H, Takahashi M, Mizuno Y, Mochizuki H
  • Journal Title: J Neurosci Volume: 32(48) Pages: 17186-17196
  • DOI: DOI:10.1523/JNEUROSCI.2220-12.2012
  • Peer Reviewed
• [Journal Article] Mitochondrial Division Ensures the Survival of Postmitotic Neurons by Suppressing Oxidative Damage (2012)
  • Author(s): Kageyama Y, Zhang Z, Roda R, Fukaya M, Wakabayashi J, Wakabayashi N, Kelsler TW, Reddy H, Iijima M, Sesaki H
  • Journal Title: J Cell Biol Volume: 197(4) Pages: 535-551
  • DOI: DOI:10.1083/jcb.201110034
  • Peer Reviewed
• [Journal Article] Pilt is a coiled-coil domain-containing protein that localizes at the trans-Golgi complex and regulates its structure (2012)
  • Author(s): Tamaki H, Sanda M, Katsumata O, Hara Y, Fukaya M, Sakagami H
  • Journal Title: FEBS lett Volume: 586(19) Pages: 3064-3070
  • DOI: DOI:10.1016/j.febslet.2012.07.051
  • Peer Reviewed
• [Presentation] Splice variant-dependent subcellular localization of the Arf6 activator BRAG2 in the adult mouse brain (2013)
  • Author(s): 深谷昌弘、阪上洋行
  • Organizer: Splice variant-dependent subcellular localization of the Arf6 activator BRAG2 in the adult mouse brain
  • Place of Presentation: 高松
  • Year and Date: 20130328-30