2012 Fiscal Year Final Research Report
Simultaneous visualization of visual responses and mono-synaptic circuits
| Project/Area Number |
23700419
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Nerve anatomy/Neuropathology
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| Research Institution | National Institute for Physiological Sciences |
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Principal Investigator |
MORI Takuma 生理学研究所, 生体情報研究系, 助教 (70545798)
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| Project Period (FY) |
2011 – 2012
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| Keywords | 神経回路網 |
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| Research Abstract |
I developed a new glycoprotein-deleted rabies virus based on a human vaccine strain, HEP-Flury. I visualized monosynaptic neuronal circuits of neocortical layer 2/3 pyramidal neurons using the virus. I electroporated plasmids into pyramidal cells in cortical layer 2/3 of mouse and injected EnvA-pseudotyped rabies virus. These neurons receive direct synaptic inputs from layers 2/3, 4 and 5. The virus enabled to observe the detail of the neuronal morphology. I analyzed the morphological characteristics of these presynaptic neurons.
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