2013 Fiscal Year Final Research Report
Identification of the transcription factors for hypothalamic orexin
| Project/Area Number |
23700458
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Neurochemistry/Neuropharmacology
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| Research Institution | Tokyo Metropolitan Institute of Medical Science |
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Principal Investigator |
TANAKA Susumu 公益財団法人東京都医学総合研究所, 精神行動医学研究分野, 主席研究員 (30399472)
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| Project Period (FY) |
2011 – 2013
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| Keywords | オレキシン / 睡眠 / 覚醒 / 転写 |
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| Research Abstract |
In this study, we tried to identify the transcription factors for hypothalamic orexin transcription.
By using One-hybrid system, we found some transcription factors binds to II3 sequence in orexin regulatory element 1. We found that one of positive clones, clone 111, expresses in embryonic lateral hypothalamus as well as orexin. Clone 111 moderates the activity of orexin upstream region in reporter assay. Clone 111 co-expresses in orexin neurons of adult hypothalamus. Clone 111 has no strong association with narcolepsy in genome wide association study.
Further studies using clone 111 knock-out mouse are needed to show the involvement of clone 111 against orexin transcription.
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[Journal Article] A prioritization analysis of disease association by data-mining of functional annotation of human genes2012
Author(s)
Taniya T, Tanaka S, Yamaguchi-Kabata Y, Hanaoka H, Yamasaki C, Maekawa H, Barrero RA, Lenhard B, Datta MW, Shimoyama M, Bumgarner R, Chakraborty R, Hopkinson I, Jia L, Hide W, Auffray C, Minoshima S, Imanishi T, Gojobori T
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Journal Title
Genomics
Volume: 99(1)
Pages: 1-9
Peer Reviewed
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