Mode of action of Septin-molecular complex

2013 Fiscal Year Final Research Report

• Project/Area Number: 23700463
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Neurochemistry/Neuropharmacology
• Research Institution: Nagoya University
• Principal Investigator: SHINODA Tomoyasu 名古屋大学, 医学(系)研究科(研究院), 助教 (80505652)
• Project Period (FY): 2011 – 2013
• Keywords: 神経発生 / 大脳皮質形成

Research Abstract

Septins are a family of conserved GTP/GDP-binding proteins implicated in a variety of cellular functions such as cell cycle control and cytokinesis. We performed a screening to indentify interacting molecules for Sept4 and Sept14. By using a method combining affinity column chromatography with shotgun liquid chromatography tandem mass spectrometry, we obtained several candidates including Drebrin-1 and Cofilin-1, regulator proteins of actin cytoskeleton. As it was previously reported that Cofilin-1 is involved in cortical neuronal migration, we examined the functional involvement of Drebrin-1 for neuronal migration. Knockdown experiments by in utero electroporation showed that reduction of Drebrin-1 caused inhibition of neuronal migration. Biochemical analyses revealed the direct interaction between Sept4 and Drebrin-1, Sept14 and Cofilin-1, respectively. These results suggest that Sept4-Sept14 complex involved in neuronal migration by regulating the dynamics of actin cytoskeleton.

Research Products

• [Journal Article] Neurogenin2-d4Venus and Gadd45g-d4Venus transgenic mice : Visualizing mitotic and migratory behaviors of cells committed to the neuronal lineage in the developing mammalian brain (2014)
  • Author(s): Kawaue T, Sagou K, Okamoto M, Shinoda T,Kiyonari H, Kawaguchi A, Miyata T
  • Journal Title: Dev. Growth Differ. Volume: (in press)
  • DOI: 10.1111/dgd.12131
  • Peer Reviewed
• [Journal Article] Ferret-mouse difference in interkinetic nuclear migration and cellular densification in the neocortical ventricular zone (2014)
  • Author(s): Okamoto M, Shinoda T, Kawaue T, Nagasaka A, Miyata T
  • Journal Title: Neuroscience Research Volume: (in press)
  • Peer Reviewed
• [Journal Article] TAG-1-assisted progenitor elongation streamlines nuclear migration to optimize subapical crowding (2013)
  • Author(s): Okamoto M, Namba T, Shinoda T, Kondo T, Watanabe T, Inoue Y, Takeuchi K, Eno moto Y, Ota K, Oda K, Wada Y, Sagou K, Sakakibara A, Kawaguchi A, Nakajima K, Adachi T, Fujimori T, Ueda M, Hayashi S, Kaibuchi K, Miyata T
  • Journal Title: Nat. Neurosci. Volume: 16
  • DOI: 10.1038/nn.3525
  • Peer Reviewed
• [Presentation] Physiological meanings of of the dynamics of the progenitor cells for the maintenance of the ventricular zone (2013)
  • Author(s): Shinoda T, et al
  • Organizer: Neuro2013
  • Place of Presentation: 京都国際会館、京都市
  • Year and Date: 2013-06-21
• [Presentation] Molecular mechanism of Septin-mediated neuronal migration (2011)
  • Author(s): 篠田友靖
  • Organizer: 第34回日本分子生物学大会
  • Place of Presentation: パシフィコ横浜、横浜市
  • Year and Date: 2011-12-08
• [Presentation] Molecular mechanism of Septin-mediated neuronal migration (2011)
  • Author(s): Shinoda T, et al
  • Organizer: Neuroscience2011
  • Place of Presentation: Washington D.C., USA
  • Year and Date: 2011-11-11
• [Presentation] Molecular mechanism of Septin-mediated neuronal migration (2011)
  • Author(s): 篠田友靖
  • Organizer: 第54回日本神経化学会
  • Place of Presentation: 山代温泉瑠璃光、金沢市
  • Year and Date: 2011-09-26
• [Book] '子宮内胎仔脳遺伝子導入法'「病気の分子形態学」日本臨床分子形態学会編 (2011)
  • Author(s): 篠田友靖、伊東秀記
  • Total Pages: 370(192-193)
  • Publisher: 学際企画株式会社