2012 Fiscal Year Final Research Report
Induction of oligodendrocytes from mouse iPS cells for transplantationinto white matter injury and association with physiological low oxygen
| Project/Area Number |
23700471
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Neurophysiology and muscle physiology
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| Research Institution | Nagoya City University |
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Principal Investigator |
MISUMI Sachiyo 名古屋市立大学, 大学院・医学研究科, 助教 (70529148)
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| Project Period (FY) |
2011 – 2012
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| Keywords | オリゴデンドロサイト / iPS 細胞 / 低酸素 / 細胞移植 / 無血清培養 / 脳室周囲白質障害 |
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| Research Abstract |
To obtain appropriate donor cells for transplantation, we differentiated oligodendrocyte (OL) lineage cells from mouse iPS cells. iPS cell-derived oligodendrocyte precursor cells (OPC) were induced effectively under serum free condition by 7-step culture method. Then we removed SSEA-1 (+) cells with MACS to prevent teratoma formation, resulting in 97% reduction. Physiological low oxygen promoted OPC proliferation with no effects for cell differentiation in vitro. iPS-derived OPCs survived in immature rat brain after transplantation, suggesting these cells are suitable for donor cells.
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