2013 Fiscal Year Final Research Report
Creation and analysis of amygdala-specific gene knockout mice utilizing a dominant-negative mutant of Cre
| Project/Area Number |
23700488
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Fusional basic brain science
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| Research Institution | The University of Tokyo |
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Principal Investigator |
KIYAMA Yuji 東京大学, 医科学研究所, 助教 (90456195)
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| Project Period (FY) |
2011 – 2013
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| Keywords | Cre recombinase / Amygdala / dominant-negative |
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| Research Abstract |
The purpose of this research is to develop a method to create mice in which the deletion of any gene of interest is highly restricted to the amygdala that subserves emotional functions. For this purpose, we tried to utilize site-specific DNA recombinase PhiC31O and a dominant-negative mutant of Cre to establish highly regional specificity. We determined that phiC31O was effective in mammalian central nervous system in conjunction with the Cre recombinase. And we created and analyzed the transgenic mice expressing Y324F mutant of Cre recombinase to verify its dominant-negative function.
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