2012 Fiscal Year Final Research Report
Development of 3D spine analysis method and analysis of spines in prefrontal cortex
| Project/Area Number |
23700496
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Fusional brain recording science
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| Research Institution | Saitama Medical University |
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Principal Investigator |
MUKAI Hideo 埼玉医科大学, 医学部, 助教 (20534358)
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| Project Period (FY) |
2011 – 2012
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| Keywords | シナプス / スパイン / 樹状突起 / 大脳皮質 / 前頭前野 / 辺縁系 / 情動 / オキシトシン |
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| Research Abstract |
We have developed a mathematical analysis program to handle vast numbers of dendritic spines in the mammalian brain, providing a better way of spine analysis for many researchers. To further enable three-dimensional analysis, we examined a number of fluorescent reagents and found a candidate substance with robust anti-fading properties suitable for confocal imaging. We also established a method for precise quantification of oxytocin in the brain and made a multi-electrode measuring system for oxytocin effects on the spines in the prefrontal cortex.
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