Generate pluripotent stem cell derived animals by using interspecific blastocyst complementation

2012 Fiscal Year Final Research Report

• Project/Area Number: 23700507
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Laboratory animal science
• Research Institution: The University of Tokyo
• Principal Investigator: KOBAYASHI Toshihiro 東京大学, 医科学研究所, 客員研究員 (20587414)
• Project Period (FY): 2011 – 2012
• Keywords: 多能性幹細胞 / 4倍体補完法 / マウス / ラット / 異種間キメラ

Research Abstract

We succeeded to generate rat embryo in mouse and mouse embryo in rat by interspecific tetraploid complementation using each pluripotent stem cell. Both of them, each PSC-derived embryo cannot survive in a xenogenic environment over some periods. However, we prove that the early development of completely PSC-derived embryo can be followed up in xenogenic environment. The system described here not only provides a powerful experimental tool for understanding of xenogenic barrier in early embryonic development but also an initial step toward the generation of PSC-derived animal in xenogenic environment.

Research Products

• [Journal Article] A retrospective analysis of germline competence in rat embryonic stem cell lines. (2013)
  • Author(s): Hirabayashi M, Tamura C, Sanbo M, Kato-Itoh M, Kobayashi T, Nakauchi H, Hochi S.
  • Journal Title: Transgenic Res. Volume: 22(2) Pages: 411-6
  • Peer Reviewed
• [Journal Article] Blastocyst complementation generates exogenic pancreas in vivo in apancreatic cloned pigs. (2013)
  • Author(s): Matsunari H, Nagashima H, Watanabe M, Umeyama K, Nakano K, Nagaya M, Kobayashi T, Yamaguchi T, Sumazaki R, Herzenberg LA, Nakauchi H.
  • Journal Title: Proc Natl Acad Sci U S A. Volume: 110(12) Pages: 4557-62
  • Peer Reviewed
• [Journal Article] Ability of tetraploid rat blastocysts to support fetal development after complementation with embryonic stem cells. (2012)
  • Author(s): Hirabayashi M, Tamura C, Sanbo M, Goto T, Kato-Itoh M, Kobayashi T, Nakauchi H, Hochi S.
  • Journal Title: Mol Reprod Dev. Volume: 79(6) Pages: 402-12
  • Peer Reviewed
• [Journal Article] Generation of kidney from pluripotent stem cells via blastocyst complementation. (2012)
  • Author(s): Usui J, Kobayashi T, Yamaguchi T, Knisely AS, Nishinakamura R, Nakauchi H.
  • Journal Title: Am J Pathol Volume: 180(6) Pages: 2417-26
  • Peer Reviewed
• [Journal Article] Identification of rat Rosa26 locus enables generation of knock-in rat lines ubiquitously expressing tdTomato. (2012)
  • Author(s): Kobayashi T, Kato-Itoh M, Yamaguchi T, Tamura C, Sanbo M, Hirabayashi M, Nakauchi H.
  • Journal Title: Stem Cells Dev. Volume: 21(16) Pages: 2981-6
  • Peer Reviewed
• [Journal Article] Development of an all-in-one inducible lentiviral vector for gene specific analysis of reprogramming. (2012)
  • Author(s): Yamaguchi T, Hamanaka S, Kamiya A, Okabe M, Kawarai M, Wakiyama Y, Umino A, Hayama T, Sato H, Lee YS, Kato-Itoh M, Masaki H, Kobayashi T, Yamazaki S, Nakauchi H.
  • Journal Title: PLoS One. Volume: 7(7) Pages: e41007
  • Peer Reviewed
• [Journal Article] Generation of germline-competent rat induced pluripotent stem cells. (2011)
  • Author(s): Hamanaka S, Yamaguchi T, Kobayashi T,Kato-Itoh M, Yamazaki S, Sato H, Umino A, Wakiyama Y, Arai M, Sanbo M, Hirabayashi M, Nakauchi H.
  • Journal Title: PLoS One. Volume: 6(7) Pages: e22008
  • Peer Reviewed
• [Journal Article] From cell therapy to organ regeneration therapy: generation of functional organs from pluripotent stem cells (2011)
  • Author(s): Nakauchi H. and Kobayashi T.
  • Journal Title: Nihon Rinsho Volume: 69(12) Pages: 2148-55
• [Presentation] GENERATION OF ROSA26-TDTOMATO KNOCK-IN RATS VIA GENE TARGETING OF PLURIPOTENT STEM CELLS (2012)
  • Author(s): Toshihiro Kobayashi, Megumi Kato-Itoh, Tomoyuki Yamaguchi, Chihiro Tamura, Makoto Sanbo, Masumi Hirabayashi, and Hiromitsu Nakauchi
  • Organizer: 10th International Society of Stem Cell Research
  • Place of Presentation: 横浜
  • Year and Date: 2012-06-15
• [Presentation] ラットRosa26遺伝子座の同定と全身性にtdTomato遺伝子を発現するノックインラットの作製 (2012)
  • Author(s): 小林俊寛,加藤-伊藤めぐみ,山口智之,田村千尋,三宝誠,平林真澄,中内啓光
  • Organizer: 第59回日本実験動物学会総会
  • Year and Date: 2012-05-26
• [Presentation] ES/iPS細胞を用いた発生工学と臓器再生への応用 (2011)
  • Author(s): 小林俊寛
  • Organizer: Advans研究会2011
  • Year and Date: 2011-12-16
• [Presentation] iPS細胞由来の臓器再生を目指して-マウス体内でラットの膵臓作製- (2011)
  • Author(s): 小林俊寛
  • Organizer: 第1回細胞再生研究会プログラム
  • Year and Date: 2011-07-31
• [Presentation] マウス体内にラットiPS細胞由来の膵臓を作製 (2011)
  • Author(s): 小林俊寛
  • Organizer: Liver2011第7回肝免疫・ウイルス・フロンティア
  • Year and Date: 2011-07-09
• [Presentation] GENERATION OF RAT EMBRYO IN MOUSE BY TETRAPLOID COMPLEMENTATION USING PLURIPOTENT STEM CELLS (2011)
  • Author(s): Kobayashi, Toshihiro, Kato-Itoh, Megumi, Yamaguchi, Tomoyuki, Hamanaka, Sanae, Hirabayashi, Masumi, Nakauchi, Hiromitsu
  • Organizer: 9th International Society of Stem Cell Research (カナダ・トロント)
  • Year and Date: 2011-06-17
• [Presentation] マウス-ラット異種間キメラの作製と再生医療への応用 (2011)
  • Author(s): 小林俊寛,中内啓光
  • Organizer: 第58回日本実験動物学会総会
  • Year and Date: 2011-05-27
• [Presentation] Generation of Rat Pancreas in Mouse via Interspecific Blastocyst Complementation Using Pluripotent Stem Cells (2011)
  • Author(s): 小林俊寛,中内啓光
  • Organizer: 第54回日本糖尿病学会総会
  • Year and Date: 2011-05-21
• [Book] 細胞工学、異種間キメラ作製技術と臓器再生への応用 (2012)
  • Author(s): 小林 俊寛, 中内 啓光
  • Total Pages: 302-307
• [Book] 多能性幹細胞を用いてマウスの体内にラットの膵臓を形成させるGeneration of Rat Pancreas in Mouse (2011)
  • Author(s): 小林俊寛, 中内啓光
  • Total Pages: 2-4
  • Publisher: Medical Science Digest
• [Book] 生物の科学遺伝、マウス生体内にラットの膵臓を作製 (2011)
  • Author(s): 小林俊寛,中内啓光
  • Total Pages: 8-15