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2012 Fiscal Year Final Research Report

Role of prosaposin in the mouse embryogenesis

Research Project

  • PDF
Project/Area Number 23700515
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Laboratory animal science
Research InstitutionTokai University

Principal Investigator

YONESHIGE Azusa  東海大学, 糖鎖科学研究所, 特定研究員 (70586750)

Project Period (FY) 2011 – 2012
Keywords胚発生
Research Abstract

Prosaposin (PSAP) is the precursor protein of saposins (SAPs) which are required for the lysosomal hydrolysis of sphingolipids. Although PSAP itselfis known to be secreted into several body fluids, its physiological function is not well understood. PSAP deficient mice (Psap-/-) are born at the much lower frequency suggesting the function of PSAP during the mouse embryogenesis. In this study, we investigated the temporal and spatial expression of PSAP in the wild-type mouse embryo and the phenotype of Psap-/-embryo. Protein levels of PSAP in the mouse embryonic tissues were low at embryonic day (E) 6.5 and increased at E7.5 to 9.5. Immunohistochemical analyses showed high expressions of PSAP in decidua at E7.5 to 9.5 and in spongiotrophoblastic layer of placenta at E10.5. SAPs were highly expressed in the large lysosomes of visceral endoderm of yolk sac. Histopathological examination of Psap-/-embryos indicated the developmental abnormality as early as at around E7.5. Shrunken cells were observed in decidua. The number and the size of large lysosome in visceral endoderm of yolk sac were decreased. These lethal phenotypes of Psap-/-embryos were rescued by maternal overexpression of PSAP. Our findings suggest that PSAP and SAPs play important roles during the early stage of mouse pregnancy, at the site of decidua, yolk sac and placenta.

  • Research Products

    (13 results)

All 2012 2011

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (7 results) Book (4 results)

  • [Journal Article] Chemoenzymatic synthesis of hydrophobic glycoprotein: synthesis of saposin C carrying complex-type carbohydrate2012

    • Author(s)
      Hojo H, Tanaka H, Hagiwara M, Asahina Y, Ueki A, Katayama H, Nakahara Y, Yoneshige A, Matsuda J, Ito Y, Nakahara Y
    • Journal Title

      Journal of OrganicChemistry

      Volume: 77 Pages: 9437-9446

    • Peer Reviewed
  • [Journal Article] Synthesis of the sphingolipid activator protein, saposin C, using an azido-protected O-acyl isopeptide as an aggregation-disrupting element2011

    • Author(s)
      Hojo H, Katayama H, Tano C, Nakahara Y, Yoneshige A, Matsuda J, Sohma Y, Kiso Y,Nakahara Y
    • Journal Title

      Tetrahedron Letters

      Volume: 52 Pages: 635-639

    • Peer Reviewed
  • [Presentation] 化学合成サポシンCのグルコシルセラミド・グルコシダーゼ活性への影響2012

    • Author(s)
      米重あづさ、北條裕信、武藤真長、松田純子
    • Organizer
      第85回日本生化学会大会
    • Place of Presentation
      福岡国際会議場
    • Year and Date
      20121214-16
  • [Presentation] プロサポシン強発現マウス胚組織の表現型解析2012

    • Author(s)
      武藤真長、米重あづさ、昼沢良介、吉村真一、松田純子
    • Organizer
      第85回日本生化学会大会
    • Place of Presentation
      福岡国際会議場
    • Year and Date
      20121214-16
  • [Presentation] The activity of chemically synthesized saposin C on glucosylceramide-・-glucosidase2012

    • Author(s)
      Yoneshige A, Hojo H, Mutou M, Matsuda J
    • Organizer
      第4回国際ライソゾーム病フォーラム
    • Place of Presentation
      東京プリンスホテル
    • Year and Date
      20121004-06
  • [Presentation] The effects of chemically synthesized saposin C on glucosylceramide-・-glucosidase2012

    • Author(s)
      Yoneshige A, Mutou M, Watanabe T, Tano C, Hojo H, Matsuda J
    • Organizer
      The 26th International Carbohydrate Symposium (ICS2012)
    • Place of Presentation
      Hotel Melia Castilla (Spain)
    • Year and Date
      20120717-22
  • [Presentation] Role of prosaposin in the embryogenesis of mouse2012

    • Author(s)
      Mutou M, Yoneshige A, Watanabe T, Matsuda J
    • Organizer
      The 26th International Carbohydrate Symposium (ICS2012)
    • Place of Presentation
      Hotel Melia Castilla (Spain)
    • Year and Date
      20120717-22
  • [Presentation] マウス胚発生におけるプロサポシンの役割2011

    • Author(s)
      武藤真長、米重あづさ、渡邉昂、松田純子
    • Organizer
      第84回日本生化学会大会
    • Place of Presentation
      国立京都国際会館
    • Year and Date
      20110922-24
  • [Presentation] Prosaposin during the embryogenesis of mouse2011

    • Author(s)
      Yoneshige A, Mutou M, Matsuda J
    • Organizer
      The 31th Naito conference
    • Place of Presentation
      Chateraise Gateaux Kingdom Sapporo
    • Year and Date
      20110913-16
  • [Book] 先天代謝異常症候群 第2版(下)-病因・病態研究、診断・治療の進歩-2012

    • Author(s)
      松田純子
    • Total Pages
      508-512
    • Publisher
      米重あづさ:サポシンA欠損症
  • [Book] 先天代謝異常症候群 第2版(下)-病因・病態研究、診断・治療の進歩-2012

    • Author(s)
      松田純子
    • Total Pages
      513-517
    • Publisher
      米重あづさ:サポシンB欠損症
  • [Book] 先天代謝異常症候群 第2版(下)-病因・病態研究、診断・治療の進歩-2012

    • Author(s)
      松田純子
    • Total Pages
      518-522
    • Publisher
      米重あづさ:サポシンC欠損症
  • [Book] ライソゾーム病-最新の病態、診断、治療の進歩2011

    • Author(s)
      松田純子
    • Total Pages
      180-183
    • Publisher
      米重あづさ:サポシン欠損症

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Published: 2014-09-25  

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