2012 Fiscal Year Final Research Report
Regulation of intercellular gap junctioncommunication intenocytes in response to mechanical st imulat ions
| Project/Area Number |
23700524
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | Hokkaido University |
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Principal Investigator |
MAEDA Eijiro 北海道大学, 大学院・工学研究院, 助教 (20581614)
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| Project Period (FY) |
2011 – 2012
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| Keywords | バイオメカニクス / メカノバイオロジー |
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| Research Abstract |
A cell culture device mimicking in vivo environment of tenocytes hasbeen developed, using MEMS fabrication techniques and PDMS. It was demonstratedusing the device that intracellular calcium ion concentration was elevated in response tophysiological mechanical stimulation(4%tensile strain or O.1 mPa fluid shear stress), inparticular that a significantly greater number of tenocytes increased the ion level whensubjected to a combined stimulation of such tensile strain and fluid shear stress, comparedto a control level. In addition, a quantitative evaluation of intercellular gap communicationwas succeeded using the current experimental model using a FLIP protocol.
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