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2012 Fiscal Year Final Research Report

Fabrication of Complex Hydrogel Materials with Microscale Patterns for Cell Organization

Research Project

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Project/Area Number 23700554
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Biomedical engineering/Biological material science
Research InstitutionChiba University

Principal Investigator

YAMADA Masumi  千葉大学, 大学院・工学研究科, 准教授 (30546784)

Project Period (FY) 2011 – 2012
Keywordsバイオマテリアル / マイクロ流路 / ハイドロゲル
Research Abstract

We developed microfluidic systems for preparing complex hydrogel materials embedding micrometer-patterns with different physicochemical properties. Complex hydrogel fibers, stripe-patterned sheets, cell-sized ECM particles, and planar patterns were fabricated. These materials were applied to, for example, the coculture of primary hepatocytes and non-parenchymal cells, the formation of heterogeneous spheroids incorporating ECM particles, and the preparation of relatively large tissues by combining these unit materials. In addition, strategies for preparing vascular tissue models have been proposed using microfluidic devices made of hydrogel.

  • Research Products

    (30 results)

All 2013 2012 2011 Other

All Journal Article (4 results) Presentation (20 results) Book (1 results) Remarks (1 results) Patent(Industrial Property Rights) (4 results)

  • [Journal Article] A Droplet-based Microfluidic Process to Produce Yarn-ball-shaped Hydrogel Microbeads2013

    • Author(s)
      A. Miyama, M. Yamada, S. Sugaya, and M. Seki
    • Journal Title

      RSC Advances

      Volume: (in press)

  • [Journal Article] Micropatterning of Hydrogels on Locally Hydrophilized Regions on PDMS by Stepwise Solution Dipping and in situ Gelation2012

    • Author(s)
      S. Sugaya, S. Kakegawa, S. Fukushima, M. Yamada, and M. Seki
    • Journal Title

      Langmuir

      Volume: 28 Pages: 14073-14080

  • [Journal Article] Controlled Formation of Heterotypic Hepatic Micro-Organoids in Anisotropic Hydrogel Microfibers for Long-Term Preservation of Liver-Specific Functions2012

    • Author(s)
      M. Yamada, R. Utoh, K. Ohashi, K. Tatsumi, M. Yamato, T. Okano, and M. Seki
    • Journal Title

      Biomaterials

      Volume: 33 Pages: 8304-8315

  • [Journal Article] Microfluidic Synthesis of Chemically and Physically Anisotropic Hydrogel Microfibers for Guided Cell Growth and Networking2012

    • Author(s)
      M. Yamada, S. Sugaya, Y. Naganuma, and M. Seki
    • Journal Title

      Soft Matter

      Volume: 8 Pages: 3122-3130

  • [Presentation] 「微細ハイドロゲル流路構造を利用した血管組織モデルの作製」2013

    • Author(s)
      山田真澄,岩瀬優輝,関実
    • Organizer
      第12回日本再生医療学会総会
    • Place of Presentation
      横浜
    • Year and Date
      20130300
  • [Presentation] マイクロフルイディクスを用いた複合ゲル材料の作製と細胞アセンブリ2012

    • Author(s)
      関実,山田真澄
    • Organizer
      日本バイオマテリアル学会シンポジウム2012
    • Place of Presentation
      仙台
    • Year and Date
      20121100
  • [Presentation] Manipulation of Cells and Cell Spheroids Using Collagen Hydrogel Microbeads Prepared by Microfluidic Devices2012

    • Author(s)
      S. Sugaya, M. Yamada, and M. Seki
    • Organizer
      2012 International Symposium on Micro-NanoMechatronics andHuman Science (MHS2012)
    • Place of Presentation
      名古屋
    • Year and Date
      20121100
  • [Presentation] Construction of Vascular Tissues via Multilayer Cell Deposition inside Hydrogel Microchannels2012

    • Author(s)
      M. Iwase, M. Yamada, and M. Seki
    • Organizer
      16th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS2012)
    • Place of Presentation
      沖縄
    • Year and Date
      20121000
  • [Presentation] Microfluidics- based Formation of Heterogeneous Hydrogel Sheets for High-density Coculture of Multiple Cell Types2012

    • Author(s)
      A. Kobayashi, K. Yamakoshi, Y. Yajima, M. Yamada, and M. Seki
    • Organizer
      16th International Conference on Miniaturized Systems for Chemistry and LifeSciences (MicroTAS2012)
    • Place of Presentation
      沖縄
    • Year and Date
      20121000
  • [Presentation] 微小コラーゲンハイドロゲル粒子を用いたヘテロ細胞集塊の作製2012

    • Author(s)
      菅谷紗里,山田真澄,関実
    • Organizer
      化学工学会第44回秋季大会
    • Place of Presentation
      仙台
    • Year and Date
      20120900
  • [Presentation] ハイドロゲル材料とマイクロフルイディクスによる細胞アセンブリの新展開2012

    • Author(s)
      山田真澄
    • Organizer
      超高速バイオアセンブラ第1回若手研究者シンポジウム
    • Place of Presentation
      京都
    • Year and Date
      20120700
  • [Presentation] マイクロノズルアレイ構造を利用した複合型細胞埋包ハイドロゲル材料の作製2012

    • Author(s)
      山田真澄,長沼洋次,小林あおい,関実
    • Organizer
      化学工学会第77年会
    • Place of Presentation
      東京
    • Year and Date
      20120300
  • [Presentation] Fluidic Preparation of Patterned Hydrogel Fibers Using Micronozzle-array Devices for Neural Cell Guidance2012

    • Author(s)
      Y. Naganuma, M. Yamada, S. Sugaya, and M. Seki
    • Organizer
      IEEE The 25th International Conference on Micro Electro Mechanical Systems (MEMS2012)
    • Place of Presentation
      パリ, フランス
    • Year and Date
      20120100
  • [Presentation] マイクロ流体システムによる細胞操作・マテリアル合成の新展開2011

    • Author(s)
      山田真澄
    • Organizer
      学振151委員会未踏・ナノデバイステクノロジーナノバイオフュージョン分科会研究会
    • Place of Presentation
      東京
    • Year and Date
      20111200
  • [Presentation] Fabrication of Complex Hydrogel Materials by Utilizing Microfluidics and Micromolding2011

    • Author(s)
      M. Yamada, Y. Naganuma, E. Yamada, S. Kakegawa, S. Sugaya, and M. Seki
    • Organizer
      2011 MRS Fall Meeting
    • Place of Presentation
      Boston, USA
    • Year and Date
      20111200
  • [Presentation] Fabrication of Functional Hydrogel Microbeads Utilizing Non-equilibrium Microfluidics for Biological Applications2011

    • Author(s)
      S. Sugaya, A. Miyama, M. Yamada, and M. Seki
    • Organizer
      2011 International Symposium on Micro-NanoMechatronics and Human Science (MHS2011)
    • Place of Presentation
      名古屋
    • Year and Date
      20111100
  • [Presentation] Microfluidics andMicrofabrication Technology for Highly PreciseCell Manipulation and Cultivation2011

    • Author(s)
      M. Yamada and M. Seki
    • Organizer
      2011International Symposium on Micro-NanoMechatronics, and Human Science (MHS2011)
    • Place of Presentation
      名古屋
    • Year and Date
      20111100
  • [Presentation] 扁平型マイクロノズルを利用した異方的ハイドゲシートの作製と細胞の高密度共培養への応用2011

    • Author(s)
      小林あおい,山田真澄,関実
    • Organizer
      第24回化学とマイクロ・ナノシステム研究会
    • Place of Presentation
      大阪
    • Year and Date
      20111100
  • [Presentation] Formation of Complex Hepatic Organoids Using Microfabricated Anisotropic Hydrogel Fibers2011

    • Author(s)
      M. Yamada, R. Utoh, K. Ohashi, M. Yamato, T. Okano, and Minoru Seki
    • Organizer
      15th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS2011)
    • Place of Presentation
      シアトル, 米国
    • Year and Date
      20111000
  • [Presentation] Microfluidic Production of Yarn-ball- shape Hydrogel Microbeads and Its Application to High-density", Cell Cultivation2011

    • Author(s)
      A. Miyama, S. Sugaya, M. Yamada, and M. Seki
    • Organizer
      15th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS2011)
    • Place of Presentation
      シアトル, 米国
    • Year and Date
      20111000
  • [Presentation] Synthesis of Complex Alginate Hydrogel Fibers Utilizing Microfluidic Devices for Controlling Cell2011

    • Author(s)
      Y. Naganuma, M. Yamada, S. Sugaya, and M. Seki
    • Organizer
      Growth Directions, International Conference on Biofabrication 2011in Toyama
    • Place of Presentation
      富山
    • Year and Date
      20111000
  • [Presentation] Synthesis of Functional Polymer/Hydrogel Materials Utilizing Non-equilibrium Microfluidics2011

    • Author(s)
      M. Yamada and M. Seki
    • Organizer
      The 14th Asian Chemical Congress (14 ACC)
    • Place of Presentation
      バンコク, タイ
    • Year and Date
      20110900
  • [Presentation] 微小ハイドロゲルファイバーを利用した肝細胞・非実質細胞複合組織体の作製2011

    • Author(s)
      山田真澄,鵜頭理恵,大橋一夫,大和雅之,岡野光夫,関実
    • Organizer
      化学工学会第43回秋季大会
    • Place of Presentation
      名古屋
    • Year and Date
      20110900
  • [Presentation] 毛糸玉状マイクロハイドロゲルビーズを担体とした高密度細胞培養2011

    • Author(s)
      三山文葵,山田真澄,菅谷紗里,関実
    • Organizer
      化学工学会第43回秋季大会
    • Place of Presentation
      名古屋
    • Year and Date
      20110900
  • [Book] 関実,マイクロフルイディクスを利用した複合型ハイドロゲル材料の作製と応用,ケミカルエンジニヤリング2011

    • Author(s)
      山田真澄
    • Total Pages
      16-20
    • Publisher
      化学工業社
  • [Remarks]

    • URL

      http://chem.tf.chiba-u.jp/gacb01/

  • [Patent(Industrial Property Rights)] 細胞評価用ハイドロゲル基材および細胞評価手法2013

    • Inventor(s)
      関 実,山田真澄,北川陽一
    • Industrial Property Rights Holder
      千葉大学
    • Industrial Property Number
      特許出願2013-028485
    • Filing Date
      2013-02-16
  • [Patent(Industrial Property Rights)] 血管組織およびその作製方法2012

    • Inventor(s)
      関 実,山田真澄,岩瀬優輝
    • Industrial Property Rights Holder
      千葉大学
    • Industrial Property Number
      特許出願2012-181261
    • Filing Date
      2012-08-18
  • [Patent(Industrial Property Rights)] 複合型肝細胞組織体およびその作製方法2011

    • Inventor(s)
      山田真澄,関 実,山田理恵,大橋 一夫,大和雅之,岡野光夫
    • Industrial Property Rights Holder
      千葉大学・東京女子医科大学
    • Industrial Property Number
      特許出願2011-122973
    • Filing Date
      2011-05-31
  • [Patent(Industrial Property Rights)] 複合型肝細胞組織体およびその作製方法2011

    • Inventor(s)
      山田真澄,関 実,山田理恵,大橋 一夫,大和雅之,岡野光夫
    • Industrial Property Rights Holder
      千葉大学・東京女子医科大学
    • Industrial Property Number
      特許出願2011-123856
    • Filing Date
      2011-06-01

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Published: 2014-09-25  

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