2013 Fiscal Year Final Research Report
Preparation of novel durable gene expression system comprising biodegradable device containing DNA complex particles coated by acidic polysaccharides
| Project/Area Number |
23700564
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | Musashino University |
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Principal Investigator |
ITO Tomoko 武蔵野大学, 薬学研究所, 客員研究員 (80372910)
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| Project Period (FY) |
2011 – 2012
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| Keywords | 遺伝子治療 / 放出制御 / ナノバイオ材料 |
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| Research Abstract |
In this study, we prepared the durable gene expression systems using injectable auto-forming alginate gel including DNA complexes coated by hyaluronic acid. When the alginate solution including the finely dispersed DNA complexes coated with hyaluronic acid was injected in a living body, a gel was formed in the injected site. The gel continuously degraded in a tumor tissue. The rate of gel-degradation and release of the DNA complexes could be controlled by addition of amorphous calcium phosphate (ACP). When the alginate solution containing the DNA complexes with cytokine-gene and ACP was injected close to the tumor tissue of the mice, the formulation showed high therapeutic effect. It is expected for applications as an injectable sustained-gene expression device.
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Research Products
(32 results)
