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2012 Fiscal Year Final Research Report

Preparation of stimuli-responsive surfaces that release cationic polymer triggered by thiol-disulfide exchange for gene introduction

Research Project

  • PDF
Project/Area Number 23700566
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Biomedical engineering/Biological material science
Research InstitutionThe University of Tokyo

Principal Investigator

AKIMOTO Aya  東京大学, 大学院・工学系研究科, 助教 (10585805)

Project Period (FY) 2011 – 2012
Keywordsバイオマテリアル
Research Abstract

In this study, we aimed to improve the efficiency of reverse transfection, one of the economic and low cytotoxic gene introduction methods, by preparing novel stimuli-responsive polycation releasing surface. Polycation release was examined by using cysteine as stimuli. As a result, polycation was released from the surface by applying cysteine containing aqueous solution which was nontoxic to cells. Moreover, 293T cells were well adhered to the surface and surface showed any cytotoxic activities. This functional surface would be potentially useful as experimental platform for gene introduction.

  • Research Products

    (1 results)

All 2013

All Journal Article (1 results) (of which Peer Reviewed: 1 results)

  • [Journal Article] Preparation of cell-culturing glass surfaces that release branched polyethyleneimine triggered by thiol-disulfide exchange2013

    • Author(s)
      Aya Mizutani Akimoto, Tohru Takarada, Mizuo Maeda
    • Journal Title

      Colloids Surf. B: Biointerfaces

      Volume: 103 Pages: 360-365

    • URL

      http://dx.doi.org/10.1016/j.colsurfb.2012.10.031

    • Peer Reviewed

URL: 

Published: 2014-09-25  

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