2013 Fiscal Year Final Research Report
Mechanical ventilation-induced diaphragmatic atrophy
| Project/Area Number |
23700781
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Sports science
|
|---|
| Research Institution | Juntendo University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2011 – 2013
|
| Keywords | 横隔膜 / 筋萎縮 / 機械的人工換気 / 温熱負荷 / 筋張力 / 熱ショックタンパク質 |
|---|
| Research Abstract |
Mechanical ventilation (MV) is used clinically to provide sufficient alveolar ventilation in patient. However, prolonged MV is associated with the development of respiratory muscle weakness. We hypothesized that exposure to heat stress would increase diaphragm expression of heat shock protein 72 (HSP72) and that this treatment would protect against MV-induced diaphragmatic atrophy. Adult male Wistar rats were randomly assigned to one of four groups: control group with no MV; 12-h controlled MV group; 1-h whole-body heat stress; or 1-h whole-body heat stress 24 h prior to 12-h controlled MV. Compared to control animals, diaphragmatic HSP72 significantly induced by heat stress. Prolonged MV resulted in significant atrophy in the costal diaphragm, however, heat stress attenuated this effect. In contrast, heat stress did not protect against MV-induced diaphragm contractile dysfunction. This heat stress-induced protection may be linked to increased expression of HSP72 in the diaphragm.
|
