2013 Fiscal Year Final Research Report

Response of intestinal macrophage to intestinal bacteria

Research Project

Project/Area Number	23700917
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Eating habits, studies on eating habits
Research Institution	Okayama Prefectural University
Principal Investigator	NAKATA Kazue 岡山県立大学, 保健福祉学部, 助教 (60411740)
Project Period (FY)	2011 – 2013
Keywords	腸管マクロファージ / 腸管感染症 / 感染防御 / 組織特異性
Research Abstract	The study aimed to clarify the mechanism of foreign substances recognition and elimination by intestinal macrophages and to identify selective intestinal macrophage responses, such as phagocytosis, bactericidal activity, and cytokine production, induced by nonpathogenic E. coli, V. parahaemolyticus, S. aureus, and L. casei. The phagocytosis of L. casei by intestinal macrophages was significantly lower than that of E. coli, V. parahaemolyticus, S. aureus. The bactericidal activities of intestinal macrophages differed for different bacterial species. TNF-alpha and IL-12 production induced by E. coli, V. parahaemolyticus, and S. aureus stimulation was significantly higher than that induced by LPS but not L. casei. IL-10 production was same for LPS and all bacteria. The production of TNF-alpha and IL-12 in intestinal macrophages may be related to phagocytosis. These results confirmed that intestinal macrophages show different responses to individual bacteria specie.