2012 Fiscal Year Final Research Report
Identification of food-derived peptide and its bioactivities after oral ingestion of hydrolysate prepared from connective tissues
| Project/Area Number |
23700944
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Eating habits, studies on eating habits
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| Research Institution | Tokyo Kasei Junior College (2012)
Osaka Yuhigaoka Gakuen College (2011) |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2012
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| Keywords | ペプチド / エラスチン / Pro-Gly / 食事由来ペプチド / プロテオグリカン |
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| Research Abstract |
In the present study, we identified prolyl-glycine (Pro-Gly) as the food-derived peptide in human blood after oral ingestion of elastin hydrolysate. Following ingestion of elastin hydrolysate (10 g/60 kg body weight) by healthy human volunteers, peripheral blood was used to prepare plasma samples from which peptides were extracted by solid phase extraction and fractionated by size-exclusion chromatography (SEC). Peptides in the SEC fractions were derivatized with phenyl isothiocyanate (PITC) and resolved by reverse phase (RP)-HPLC. Pro-Gly was the major food-derived elastin peptide, reaching a maximum (18 μM) at 30 minutes after ingestion, and decreasing approximately20% at 4 hours after ingestion. Finally, in cell culture, levels of Pro-Gly in the medium above 1 μg/mL significantly enhanced elastin synthesis of normal human dermal fibroblasts (NHDF) without affecting the rate of cell proliferation.
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[Journal Article] Identification of Food-Derived Elastin Peptide, Prolyl-Glycine (Pro-Gly),in Human Blood after Ingestion of Elastin Hydrolysate.2012
Author(s)
Shigemura, Y.; Nakaba, M.; Shiratsuchi,E.; Suyama, M.; Yamada, M.; Kiyono, T.;Fukamizu, K.; Park, E. Y.; Nakamura, Y.;Sato, K.
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Journal Title
Journal of agricultural and food chemistry
Volume: 60 (20)
Pages: 5128-5133
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