2012 Fiscal Year Final Research Report
Phosphoproteomic analysis of clinical colon cancer specimen and validation by SRM/MRM analysis.
| Project/Area Number |
23701093
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Tumor diagnosis
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| Research Institution | 独立行政法人医薬基盤研究所 |
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Principal Investigator |
SHIROMIZU Takashi 独立行政法人医薬基盤研究所, 創薬基盤研究部, 研究員 (00582678)
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| Project Period (FY) |
2011 – 2012
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| Keywords | 大腸癌 / プロテオミクス / バイオマーカー |
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| Research Abstract |
We performed mass spectrometry-based quantitative phosphoproteomic analysis to search for biomarker candidates by using surgically extracted colorectal cancer tissues. Novel biomarker candidates were identified by optimization of analysis conditions. For the selection of candidate genes, pathway analysis and comparison with the results of metastatic cancer cell lines were performed. Also by in vitro invasion assay, we have identified a novel functional molecule involved in cancer invasion.
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