2013 Fiscal Year Final Research Report
Identification of the cell type specific region for retroviral vector integration
| Project/Area Number |
23701099
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Clinical oncology
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| Research Institution | Shinshu University |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2013
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| Keywords | 遺伝子治療 |
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| Research Abstract |
Retroviral vector integration near the LMO2 promotor is the most likely cause of leukemogenesis in the gene therapy trails. Here, we showed identification of a high incidence region (HIR) for retroviral vector integration, which we had originally identified in the LMO2 promotor region, near the promotor region of the CCND2 locus, suggesting that there is the HIR in the promotor region of various genes. To perform comprehensive analysis of HIR, next-generation sequencer was applied to examine the analysis condition for the HIR. Furthermore, using the next-generation sequencer, 600 candidates for proto-oncogene or the cell proliferation-related gene, which are targets for the vector integration on HIR, were identified.
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