2013 Fiscal Year Final Research Report
PREPARATION OF SMALL PLASMID COMPLEX FOR CANCER THERAPY AND APPLICATION TO MEDIUM ANIMAL CLINICAL STUDY
| Project/Area Number |
23701103
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Clinical oncology
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| Research Institution | Otsuma Women's University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
KOYAMA Yoshiyuki 大妻女子大学, 家政学部, 教授 (00162090)
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| Project Period (FY) |
2011 – 2013
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| Keywords | 遺伝子治療 |
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| Research Abstract |
We have established a DNA/PEI/chondroitin sulfate ternary complex system which mediates highly efficient tumor transfection in the living body.In this study, we found that transfection of the tumor cells with a pathogenic gene could effectively enhance the anti-tumor immunity. Those antigen expressed on the tumor cell surface would be recognized by APCs as a danger signal to provoke the immune response. We prepared the plasmid encoding pathogenic genes. Tumor-bearing mice were prepared, and transfected with those plasmids to examine the anti-tumor therapeutic effects. Transfection of the viral or bacterial antigenic protein genes showed highly effective anti-tumor activity. Co-transfection of the cytokine-genes with the pathogenic antigen-gene showed much higher anti-tumor therapeutic effect than pathogenic- orcytokine-genes alone.Transfection of the pathogenic gene was effective also on the primary tumor in dog, and apparent suppression of the tumor growth was observed.
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