2012 Fiscal Year Final Research Report
Swift exploration of the receptor binding characteristics of low molecular weight endocrine disruptors for orphan nuclear receptors
| Project/Area Number |
23710080
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Risk sciences of radiation/Chemicals
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| Research Institution | Kyushu University |
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Principal Investigator |
LIU Xiaohui 九州大学, 理学研究院, 学術研究員 (60596849)
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| Project Period (FY) |
2011 – 2012
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| Keywords | 有害化学物質 / 蛋白質 / 脳・神経 / 生理活性 / ビスフェノールA |
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| Research Abstract |
With no radiolabeled standard compound for the receptor-binding assay, it is difficult to make a structure-activity study especially for "orphan nuclear receptor." The SPR method requires no standard compound in receptor assay, and thus in this study, we intended to establish a novel way to perform SPR prior to the ordinary binding assay. By using non-radiolabeled compounds, we carried out SPR to explore the ligands of particular nuclear receptor, and those that bind to the receptor were then utilized for radio-ligand receptor binding assay. In several occasions, we could find out eventually a very specific chemical that strongly binds to a certain important nuclear receptor.
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[Journal Article] Bisphenol-binding pocket of constitutively active nuclear receptor car: docking modeling for close-packing2013
Author(s)
Matsuyama, Y., Liu, X., Nishimura, H.,Matsushima, A., Nose, T., andShimohigashi, Y
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Journal Title
Peptide Science 2012
Pages: 401-402
Peer Reviewed
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[Journal Article] The impact of bisphenol A-feeding on the Drosophila neuropeptide PDF mRNA structure2013
Author(s)
Matsuo, A., Nakamura, M., Takeda, Y., Matsuyama, Y., Sumiyoshi, M., Liu, X., Matsushima, A., Shimohigashi, M., and Shimohigashi, Y
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Journal Title
Peptide Science 2012
Pages: 75-76
Peer Reviewed
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[Journal Article] Fine spatial assembly for construction of the phenol-binding pocket to capture bisphenol A in the human nuclear receptor ERR2012
Author(s)
Liu, X., Matsushima, A., Nakamura, M., Costa, T., Nose, T., and Shimohigashi, Y
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Journal Title
Journal of Biochemistry
Volume: 151
Pages: 403-415
DOI
Peer Reviewed
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