A study on genotoxicity of nanomaterials using mammalian cultured cells

2012 Fiscal Year Final Research Report

• Project/Area Number: 23710081
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Risk sciences of radiation/Chemicals
• Research Institution: Osaka Prefecture University
• Principal Investigator: KAWANISHI Masanobu 大阪府立大学, 大阪府立大学, 助教 (70332963)
• Project Period (FY): 2011 – 2012
• Keywords: ナノマテリアル / ナノマグネタイト / 遺伝毒性 / 培養細胞 / 小核試験 / 姉妹染色分体交換試験 / DNA二重鎖切断 / 活性酸素種

Research Abstract

We examined the genotoxicity of magnetite nanoparticles (primary particle size: 10 nm) on human A549 and Chinese hamster ovary (CHO) AA8 cells. Six hours’ treatment with the particles dose-dependently increased the frequency of micronuclei (MN) in the A549 and CHO AA8 cells up to 5.2% and 5.0% at a dose of 200 μg/mL (34 μg/cm2), respectively. In A549 cells, treatment with the nanoparticles (2 μg/mL) for 1 hour induced H2AX phosphorylation, which is suggestive of DNA double strand breaks (DSB). Treating CHO AA8 cells with 2 μg/mL magnetite for 1 hour resulted in a five times higher frequency of sister chromatid exchange (SCE) than the control level. We detected reactive oxygen species (ROS) in CHO cells treated with the particles. These findings indicate that magnetite nanoparticles induce ROS in mammalian cells, leading to the direct or indirect induction of DSB, followed by clastogenic events including MN and SCE.

Research Products

• [Journal Article] Genotoxicity and reactive oxygenspecies production induced by magnetitenanoparticles in mammalian cells (2013)
  • Author(s): Masanobu Kawanishi, Sayaka Ogo, MihoIkemoto, Yukari Totsuka, Kousuke Ishino,Keiji Wakabayashic Takashi Yagi
  • Journal Title: Journalof Toxicological Sciences Volume: 38(3)
  • Peer Reviewed
• [Journal Article] Genotoxicity of multi-walled carbonnanotubes in both in vitro and in vivoassay systems (2013)
  • Author(s): Tatsuya Kato, YukariTotsuka, Kousuke Ishino, Yoko Matsumoto,Yukie Tada, Dai Nakae, Sumio Goto, ShuichiMasuda, Sayaka Ogo, Masanobu Kawanishi,Takashi Yagi, Tomonari Matsuda, MasatoshiWatanabe, Keiji Wakabayashi
  • Journal Title: Nanotoxicology Volume: 7 Pages: 452-61
  • DOI: doi:10.3109/17435390.2012.674571
  • Peer Reviewed
• [Presentation] カオリンの遺伝毒性発現メカニズムの解明 (2012)
  • Author(s): 池本実穂, 川西優喜, 戸塚ゆ加里,若林敬二, 八木孝司
  • Organizer: 日本環境変異原学会第41回大会
  • Place of Presentation: 静岡
  • Year and Date: 20121129-30
• [Presentation] Genotoxicity of nanomaterialsmediated by ROS generation in vitro (2012)
  • Author(s): MihoIkemot, Masanobu Kawanishi, YukariTotsuka, Keiji Wakabayashi, Takashi Yagi
  • Organizer: 3rd Asian Conference of EnvironmentalMutagens
  • Place of Presentation: China
  • Year and Date: 20121023-26
• [Presentation] ナノ材料による小核誘導における相同組換え修復の関与 (2011)
  • Author(s): 池本実穂, 川西優喜,戸塚ゆ加里, 若林敬二, 八木孝司
  • Organizer: 日本環境変異原学会第40回大会
  • Place of Presentation: 東京
  • Year and Date: 20111121-22
• [Presentation] Genotoxicity induced by nanoparticles (2011)
  • Author(s): Yukari Totsuka, Tatsuya Kato, KousukeIshino, Shuichi Masuda, Dai Nakae, YukieTada, Masanobu Kawanishi, Takashi Yagi,Masatoshi Watanabe, Keiji Wakabayashi,Hitoshi Nakagama
  • Organizer: 41st European Environmental Mutagen Society annualconference
  • Place of Presentation: Barcelona, Spain
  • Year and Date: 20110704-07