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2013 Fiscal Year Final Research Report

A study on metabolic pathway of fullerene C60 in vivo.

Research Project

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Project/Area Number 23710083
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Risk sciences of radiation/Chemicals
Research InstitutionNational Institute of Health Sciences

Principal Investigator

KUBOTA Reiji  国立医薬品食品衛生研究所, 生活衛生化学部, 主任研究官 (80392299)

Project Period (FY) 2011 – 2013
Keywordsフラーレン / 代謝物 / LC/MS/MS / in vitro / 細胞毒性 / 肝ミクロゾーム
Research Abstract

For the determination of fullerene derivative (C60O, C60O2, C60O3, and C60(OH) 2) with the possibility of existing as a metabolite of C60 in vivo, a sensitive analytical method using high performance liquid chromatography – tandem mass spectrometry (LC/MS/MS) and extraction procedure from samples of in vitro studies was established in this study. We examined the cytotoxic effects of fullerene derivative with HepG2 cells. C60O induced cytotoxic effects in HepG2 cells and the tendency was enhanced in a concentration–dependent manner. On the other hand, C60O2 and C60O3 were less toxic than C60. We evaluated the metabolism of fullerene in vitro by using rat liver microsomes. No fullerene derivatives were observed in this metabolic testing system.

  • Research Products

    (7 results)

All 2012 2011 2010

All Journal Article (2 results) Presentation (5 results)

  • [Journal Article] Sub-acute oral toxicity study with fullerene C60 in rats2012

    • Author(s)
      Takahashi, M., Kato, H., Doi, Y., Hagiwara, A., Hirata-Koizumi, M., Ono, A., Kubota, R., Nishimura, T., Hirose, A.
    • Journal Title

      The Journal of Toxicological Sciences

      Volume: 37, 2 April Pages: 353-361

  • [Journal Article] Time-dependent variation in the biodistribution of C60 in rats determined by liquid chromatography-tandem mass spectrometry2011

    • Author(s)
      Kubota, R., Tahara, M., Shimizu, K., Sugimoto, N., Hirose, A., Nishimura T.
    • Journal Title

      Toxicology Letters

      Volume: 206 Pages: 172-177

  • [Presentation] Time-dependent biodistribution of C60 in rat after tail-vein administration2011

    • Author(s)
      Kubota, R., Tahara, M., Shimizu, K., Kobayashi, N., Sugimoto, N., Hirose, A., Nishimura T.
    • Organizer
      47th Congress of the European Societies of Toxicology, abstracts.
    • Place of Presentation
      Paris, France(2342)
    • Year and Date
      20110800
  • [Presentation] Bio-distribution of C60 fullerene injected into the rat tail vein2011

    • Author(s)
      Nishimura, T., Kubota, R., Tahara, M., Shimizu, K., Obama, T., Hirose, A., Sugimoto, N.
    • Organizer
      5th International Symposium on Nanotechnology, Occupational and Environmental Health (NanOEH)
    • Place of Presentation
      Boston, USA(abstracts)
    • Year and Date
      20110800
  • [Presentation] Bio-distribution of fullerenes intravenous administration in rat2011

    • Author(s)
      Nishimura, T., Kubota, R., Tahara, M., Shimizu, K., Obama, T., Sugimoto, N., Hirose, A.
    • Organizer
      50th Anniversary Annual Meeting & ToxExpo, abstracts
    • Place of Presentation
      SOT, Washington, D.C., USA
    • Year and Date
      20110300
  • [Presentation] Bio-distribution of fullerenes intravenous administration2010

    • Author(s)
      Nishimura, T., Kubota, R., Tahara, M., Shimizu, K., Obama, T., Sugimoto, N., Hirose, A., Ikarashi, Y.
    • Organizer
      2010 Annual Meeting of the Korea Society of Environmental Health and Toxicology, abstracts
    • Place of Presentation
      Suwon, Korea
    • Year and Date
      20101100
  • [Presentation] Tissue distribution of fullerene after injected into rat tail vein to mimic the absorption from the digestive tract2010

    • Author(s)
      Nishimura, T., Kubota, R., Shimizu, K., Tahara, M., Obama, T., Sugimoto, N., Kanno, J., Hirose, A.
    • Organizer
      XII International Congress of Toxicology, abstracts. P303-024
    • Place of Presentation
      Barcelona, Spain
    • Year and Date
      20100700

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Published: 2015-06-25  

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