2013 Fiscal Year Final Research Report
Formation of DNA adducts and mechanism of mutagenesis induced by nanomaterials
| Project/Area Number |
23710084
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Risk sciences of radiation/Chemicals
|
|---|
| Research Institution | Nippon Medical School (2013)
National Center of Neurology and Psychiatry (2011-2012) |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2011 – 2013
|
| Keywords | DNA付加体 / 突然変異 / ナノマテリアル / 酸化ストレス / LC-MS/MS / アダクトミクス / マグネタイト / アダクトーム解析 |
|---|
| Research Abstract |
Nanomaterials are useful owing to their characteristic properties and are commonly used in various fields. Nanosized magnetite (MGT) is widely utilized in medicinal and industrial fields; however, their toxicological properties are not well studied. In our previous study, we found that DNA damage analyzed by the comet assay of the lungs of ICR mice intratracheally instilled with a single dose of MGT at 0.05 or 0.2 mg/animal was approximately two- to three-fold greater than that of vehicle-instilled ICR mice. In this study, a comprehensive DNA adduct analysis (DNA adductome) revealed that a broad array of DNA adducts, including etheno-dA and heptanone-etheno-dC, were produced and that most of the DNA adducts determined as the major contributors in the MGT-exposed group could be derived from inflammation. These findings suggest that inflammatory responses are probably involved in the genotoxicity induced by MGT in the lungs of mice.
|
Research Products
(10 results)
-
-
[Journal Article] Genotoxicity of multi-walled carbon nanotubes in both in vitro and in vivo assay systems2013
Author(s)
Kato T, Totsuka Y, Ishino K, Matsumoto Y, Tada Y, Nakae D, Goto S, Masuda S, Ogo S, Kawanishi M, Yagi T, Matsuda T, Watanabe M, Wakabayashi K
-
Journal Title
Nanotoxicology
Volume: 7
Pages: 452-461
DOI
-
-
-
-
-
-
-
-
