DNA methylation analysis by surface plasmon resonance on a microhole array

2012 Fiscal Year Final Research Report

• Project/Area Number: 23710152
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Microdevices/Nanodevices
• Research Institution: National Institute of Advanced Industrial Science and Technology
• Principal Investigator: KURITA Ryoji 独立行政法人産業技術総合研究所, バイオメディカル研究部門, 主任研究員 (50415676)
• Project Period (FY): 2011 – 2012
• Keywords: DNA / メチルシトシン / エピジェネティクス

Research Abstract

We report the sequence-selective discrimination of the cytosine methylation status in DNA with anti methylcytosine antibody for the first time. This was realized by employing an affinity measurement involving the target methylcytosine in a bulge region an

Research Products

• [Journal Article] DNA Methylation Analysis Triggered by Bulge Specific Immuno-Recognition (2012)
  • Author(s): Ryoji Kurita and Osamu Niwa
  • Journal Title: Analytical Chemistry Volume: 84 Pages: 7533-7538
  • DOI: DOI:10.1021/ac301702y
• [Journal Article] Determination of DNA Methylation using Electrochemiluminescence with Surface Accumulable Coreactant (2012)
  • Author(s): Ryoji Kurita, Kumi Arai, Kohei Nakamoto, Dai Kato and Osamu Niwa
  • Journal Title: Analytical Chemistry Volume: 84 Pages: 1799-1803
  • DOI: DOI:10.1021/ac202692f
• [Presentation] DNA バルジを利用したシーケンス選択的なメチルシトシン免疫測定法 (2013)
  • Author(s): 栗田僚二、丹羽修
  • Organizer: 電気化学会
  • Place of Presentation: 東北大学(宮城県)
  • Year and Date: 2013-03-31
• [Presentation] Single methylation analysis in DNA by electrochemiluminescence and surface plasmon resonance (2013)
  • Author(s): Ryoji Kurita
  • Organizer: Pittcon2013
  • Place of Presentation: Pennsylvania Convention Center Philadelphia(米国)
  • Year and Date: 2013-03-20
• [Presentation] DNA バルジ特異的な抗体認識を利用したシトシンのメチル化診断 (2012)
  • Author(s): 栗田僚二、丹羽修
  • Organizer: 分析化学会
  • Place of Presentation: 金沢大学(石川県)
  • Year and Date: 2012-09-19
• [Book] メチル化 DNA の迅速検出を目指して (2013)
  • Author(s): 栗田僚二
  • Total Pages: 8-15
  • Publisher: 化学とマイクロ・ナノシステム研究会誌
• [Remarks]
  • URL: http://unit.aist.go.jp/biomed-ri/biomed-nbd/menber_kurita/kurita.html
• [Patent(Industrial Property Rights)] メチルシトシン検出法 (2013)
  • Inventor(s): 栗田僚二、丹羽修
  • Industrial Property Rights Holder: 栗田僚二、丹羽修
  • Industrial Property Number: 特願 2013-051501
  • Filing Date: 2013-03-14
• [Patent(Industrial Property Rights)] メチル化核酸検出法 (2011)
  • Inventor(s): 栗田僚二、加藤大、丹羽修
  • Industrial Property Rights Holder: 栗田僚二、加藤大、丹羽修
  • Industrial Property Number: 特願 2011-098844
  • Filing Date: 2011-04-27