Identification of allele-specific methylation regions involved in congenital genomic imprinting disorders

2013 Fiscal Year Final Research Report

• Project/Area Number: 23710223
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Medical genome science
• Research Institution: Tohoku University
• Principal Investigator: HIURA Hitoshi 東北大学, 医学(系)研究科(研究院), 助教 (70451523)
• Project Period (FY): 2011 – 2013
• Keywords: 生殖補助医療 / インプリント

Research Abstract

Recently, assisted reproductive technologies (ART) have become available to infertile couples who wish to have children. However, reports of rare imprinting disorders have been increased around the globe with the popularization of assisted reproductive technologies. In this study, to clarify the influence of ART for genomic imprinting and identify the ART-related risk factor(s), we analyzed the DNA methylation status of 22 differentially methylated regions in BWS and SRS patients. We observed a more widespread disruption of genomic imprints after ART. Our data suggest that ART-related risk factor(s) was a process after the fertilization than the gamete formative period.

Research Products

• [Journal Article] Stability of the abnormal imprinting of human induced pluripotent stem cells (2013)
  • Author(s): Hiura H, Toyoda M, Okae H, Sakurai M, Miyauchi N, Sato A, Kiyokawa N, Okita H, Miyagawa Y, Akutsu H, Nishino K, Umezawa A, Arima T
  • Journal Title: BMC Genetics Volume: 14 Pages: 32
  • DOI: 10.1186/1471-2156-14-32
  • Peer Reviewed
• [Journal Article] DNA methylation errors in imprinting disorders and assisted reproductive technologies (2013)
  • Author(s): Chiba H, Hiura H, Okae H, Miyauchi N, Sato F, Sato A, Arima T
  • Journal Title: Pediatrics international Volume: 55 Pages: 542-549
  • DOI: 10.1111/ped.12185
  • Peer Reviewed
• [Journal Article] Aberrant DNA methylation of imprinted loci in male and female germ cells of infertile couples (2012)
  • Author(s): Arima T,Okae H, Hiura H, Miyauchi N, Sato F, Sato A, Hayashi C
  • Journal Title: DNA METHYLATION INTECH Volume: 29 Pages: 183-192
  • URL: http://www.intechopen.com/books/dna-methylation-from-genomics-to-technology
  • Peer Reviewed
• [Journal Article] High-throughput detection of aberrant imprint methylation in the ovarian cancer by the bisulphite PCR-Luminex method (2012)
  • Author(s): Hiura H, Okae H, Kobayashi H, Miyauchi N, Sato F, Sato A, Suzuki F, Nagase S, Sugawara J, Nakai K, Yaegashi N, Arima T
  • Journal Title: BMC Medical Genomics Volume: 5 Pages: 8-17
  • DOI: 10.1186/1755-8794-5-8
  • Peer Reviewed
• [Journal Article] Characterization of DNA methylation errors in patients with imprinting disorders conceived by assisted reproductive technologies (2012)
  • Author(s): Hiura H, Okae H, Miyauchi N, Sato F, Sato A, Van De Pette M, John R M, Kagami M, Nakai K, Soejima H, Ogata T, Arima T
  • Journal Title: Human Reproduction Volume: 27(8) Pages: 2541-2548
  • DOI: 10.1093/humrep/des197
  • Peer Reviewed