2012 Fiscal Year Final Research Report
Development of a novel protocol based enrichment of glycopeptides for comprehensive glycoproteomics
| Project/Area Number |
23710246
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Living organism molecular science
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| Research Institution | 公益財団法人野口研究所 (2012)
Hokkaido University (2011) |
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Principal Investigator |
KUROGOCHI Masaki 公益財団法人野口研究所, 研究部, 研究員 (30422009)
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| Project Period (FY) |
2011 – 2012
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| Keywords | プロテオミクス / グライコプロテオミクス |
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| Research Abstract |
Separation of neutral and acidic glycopeptides has been developed using DEAE-Sepharose which has two characteristics, hydrophilic interaction (HILIC) and weak ion-exchange. By this method, neutral, sialylated, sulfated glycopeptides from human apolipoprotein B-100, bovine thyroglobulin could be isolated.
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