2013 Fiscal Year Final Research Report
Gene regulation by using an ligand-responsive miRNA pathway
| Project/Area Number |
23710270
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Chemical biology
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| Research Institution | Osaka University |
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Principal Investigator |
MURATA Asako 大阪大学, 産業科学研究所, 助教 (50557121)
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| Project Period (FY) |
2011 – 2012
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| Keywords | マイクロRNA / cell-basedセレクション / 低分子化合物 |
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| Research Abstract |
This study aimed to explore an artificial miRNA pathway that can be regulated by small-molecule ligands. Ligand-binding sites were created in an endogenous pre-miRNA by partially randomizing the sequence. Construction of the expression vector containing the partially randomized pre-miRNA library and the optimization of conditions for cell-based selection were performed. Moreover, in vitro selection of RNA molecules that bind to a novel naphthyridine derivatives was performed.
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